Yang D L, Hao L J, Ni D S, Zhou N
J Tongji Med Univ. 1989;9(1):13-7. doi: 10.1007/BF02933737.
SDS-polyacrylamide gel electrophoresis and Western blotting analysis by using 9 clones of monoclonal antibodies specific for hemorrhagic fever with renal syndrome (HFRS) viruses were carried out on the virion proteins of 17 strains of HFRS viruses isolated from different areas in Asia and different hosts such as Apodemus agrarius, Rattus norvegicus, domestic cat and HFRS patients. Polypeptide with apparent molecular weight about 50 kitodalton (Kd) could be detected in all 17 strains of HFRS viruses. On this polypeptide there are two different antigenic determinants and one of them might be genus specific.
利用9株对肾综合征出血热(HFRS)病毒特异的单克隆抗体,对从亚洲不同地区以及黑线姬鼠、褐家鼠、家猫和HFRS患者等不同宿主分离出的17株HFRS病毒的病毒粒子蛋白进行了SDS-聚丙烯酰胺凝胶电泳和蛋白质印迹分析。在所有17株HFRS病毒中均可检测到表观分子量约为50千道尔顿(Kd)的多肽。在该多肽上存在两种不同的抗原决定簇,其中之一可能是属特异性的。