Jones S R, Woo P T
Department of Zoology, University of Guelph, Ontario, Canada.
J Wildl Dis. 1989 Jul;25(3):413-5. doi: 10.7589/0090-3558-25.3.413.
The sensitivities of three techniques used for detecting infections of Trypanosoma spp. in frogs (Rana spp.) were compared. In total, 52 of 99 frogs had detectable infections of T. rotatorium, T. chattoni, T. pipientis or T. ranarum. Two or more Trypanosoma spp. were detected in 12 frogs. Microscopic examination of stained kidney impressions (KIT) was more sensitive than either hematocrit centrifugation (HCT) or wet-mount examination (WME) in detecting T. rotatorium and T. chattoni. The HCT was more sensitive in detecting T. pipientis and T. ranarum. Four infections of T. rotatorium that were missed using the HCT were detected using the WME; one of these was missed using the KIT. Success of the KIT may be related to size of the trypanosome while success of the HCT may be related to size, motility or specific gravity of the trypanosome.
比较了用于检测蛙（蛙属）锥虫属感染的三种技术的敏感性。总共99只蛙中有52只检测到有旋毛虫、查顿锥虫、皮氏锥虫或蛙锥虫感染。在12只蛙中检测到两种或更多种锥虫属。在检测旋毛虫和查顿锥虫时，对染色肾脏压片进行显微镜检查（KIT）比血细胞比容离心法（HCT）或湿片检查（WME）更敏感。HCT在检测皮氏锥虫和蛙锥虫时更敏感。使用WME检测到4例使用HCT漏检的旋毛虫感染；其中1例使用KIT漏检。KIT的成功率可能与锥虫的大小有关，而HCT的成功率可能与锥虫的大小、运动性或比重有关。
