Holländer H, Egensperger R, Dirlich G
Max-Planck-Institut für Psychiatrie, Planegg-Martinsried, F.R.G.
J Neurosci Methods. 1989 Jul;29(1):1-4. doi: 10.1016/0165-0270(89)90102-7.
Rhodamine-labelled latex microspheres (RLM), 10-110 nm in diameter, were used as a retrograde tracer in cultured sympathetic neurons. Retrogradely transported RLM were isolated from the cells and measured. These were all smaller than 50 nm in diameter and represented not more than 2% of the original RLM population. The permeability of RLM as a function of size was also estimated and showed a steep decrease between 20 and 30 nm from 100% to less than 20%. Therefore, RLM with a mean diameter of 20 nm would be optimal for retrograde tracing.
