Azboy Ibrahim, Demirtaş Abdullah, Bulut Mehmet, Alabalik Ulaş, Uçar Yavuz, Alemdar Celil
Department of Orthopedics and Traumatology, Dicle University, Faculty of Medicine, Diyarbakır, Turkey.
Department of Pathology, Dicle University, Faculty of Medicine, Diyarbakır, Turkey.
Acta Orthop Traumatol Turc. 2014;48(2):212-6. doi: 10.3944/AOTT.2014.13.0075.
The aim of this study was to evaluate the effects of the antithrombotic agents enoxaparin and rivaroxaban on tissue survival following skin degloving injury in an experimental rat tail model.
The study included 24 rats divided into three equal groups of 8; the enoxaparin group (Group 1), the rivaroxaban group (Group 2) and the saline control group (Group 3). A degloving injury was created by making a circular incision 5 cm distal to the base of the tail; manual traction was applied to the tail skin distal to the incision. After 15 minutes, the ends of the incision were sutured back in place. Antithrombotic agents were administered immediately after suturing and repeated once a day for 15 days. At the end of Day 15, the experiment was terminated. Gross morphological tissue survival and histopathology were evaluated.
Histopathological examination of the enoxaparin and rivaroxaban groups revealed that the skin was mostly normal or intact with minimal inflammation. The mean length of necrotic area was significantly higher in the saline group compared to the enoxaparin and rivaroxaban groups (p<0.05). No statistically significant differences were noted between the rivaroxaban and enoxaparin groups (p=0.451). The mean extent of skin necrosis was significantly higher in the control group than the study groups (p<0.05), while there was no significant difference in the length of necrotic area between Group 1 and 2 (p=0.722).
Rivaroxaban and enoxaparin improved tissue survival in skin degloving injuries in terms of gross morphological and histopathological findings in a rat tail model.
本研究旨在评估抗血栓药物依诺肝素和利伐沙班对实验性大鼠尾部皮肤脱套伤后组织存活的影响。
本研究纳入24只大鼠，平均分为三组，每组8只；依诺肝素组（第1组）、利伐沙班组（第2组）和生理盐水对照组（第3组）。在距尾根部5 cm处做环形切口造成皮肤脱套伤；对切口远端的尾部皮肤施加手动牵引。15分钟后，将切口两端缝合回原位。缝合后立即给予抗血栓药物，每天重复给药一次，共给药15天。在第15天结束时，终止实验。评估大体形态学组织存活情况和组织病理学。
依诺肝素组和利伐沙班组的组织病理学检查显示，皮肤大多正常或完整，炎症轻微。与依诺肝素组和利伐沙班组相比，生理盐水组的坏死区域平均长度显著更高（p<0.05）。利伐沙班组和依诺肝素组之间未观察到统计学显著差异（p=0.451）。对照组的皮肤坏死平均范围显著高于研究组（p<0.05），而第1组和第2组之间的坏死区域长度无显著差异（p=0.722）。
在大鼠尾部模型中，从大体形态学和组织病理学结果来看，利伐沙班和依诺肝素可改善皮肤脱套伤后的组织存活情况。
