Sung Kyunghwa, Kim Minjeong, Hyun Jiyoung, Kim Younghee, Kim Kisok
College of Pharmacy, Keimyung University, Daegu 704-701, Republic of Korea.
College of Pharmacy, Keimyung University, Daegu 704-701, Republic of Korea; College of Pharmacy, Ewha Womans University, Seoul 151-742, Republic of Korea.
Neurosci Lett. 2014 May 21;569:158-62. doi: 10.1016/j.neulet.2014.04.005. Epub 2014 Apr 18.
Although exposure to olfactory stimulants can induce neurobehavioral changes, there is a lack of data regarding specific effects on neurotransduction, especially nitric oxide (NO)-mediated neurotransduction. We investigated the relationship between altered behavioral performance and changes in the expression of nitric oxide synthase (NOS), nNOS, iNOS, and eNOS, in 2-methylbutyric (2MB) acid-exposed mice. Mice were exposed to 2MB acid by inhalation and olfactory preference/avoidance and motor coordination were measured. Additionally, we examined NOS expression in the olfactory bulb of the mouse brain. Mice exposed to 2MB acid showed significant changes in olfactory preference and rotarod activity as compared with controls. Although there was no change in nNOS and iNOS expressions in the olfactory bulb of 2MB acid-exposed mice, eNOS expression increased significantly in the olfactory bulb of 9.0M 2MB acid-exposed mice. These data indicate that altered eNOS expression in the olfactory bulb may contribute to 2MB acid-induced behavioral changes.
尽管接触嗅觉刺激物可诱发神经行为变化，但关于其对神经传导，尤其是一氧化氮（NO）介导的神经传导的具体影响的数据却很缺乏。我们研究了暴露于2-甲基丁酸（2MB）的小鼠行为表现改变与一氧化氮合酶（NOS）、神经元型NOS（nNOS）、诱导型NOS（iNOS）和内皮型NOS（eNOS）表达变化之间的关系。通过吸入使小鼠暴露于2MB酸，并测量嗅觉偏好/回避和运动协调性。此外，我们检测了小鼠脑嗅球中的NOS表达。与对照组相比，暴露于2MB酸的小鼠在嗅觉偏好和转棒试验活动中表现出显著变化。尽管暴露于2MB酸的小鼠嗅球中nNOS和iNOS表达没有变化，但在暴露于9.0M 2MB酸的小鼠嗅球中，eNOS表达显著增加。这些数据表明，嗅球中eNOS表达的改变可能导致2MB酸诱导的行为变化。
