Lodz University of Technology, Institute of General Food Chemistry, Stefanowskiego 4/10, 90-924 Lodz, Poland.
Centre of Molecular and Macromolecular Studies in Lodz, Sienkiewicza 112, 90-363 Lodz, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Aug 14;129:320-5. doi: 10.1016/j.saa.2014.03.039. Epub 2014 Apr 2.
Preliminary results of a study of the interaction between 3-amino phenylboronic acid and glucose or ZnS:Cu quantum dots are presented in this paper. ZnS:Cu quantum dots with mercaptopropionic acid as a capping agent were obtained and characterized. Quenching of 3-amino phenylboronic acid fluorescence was studied by steady-state and timeresolved measurements. For fluorescence quenching with glucose the results of steady-state measurements fulfill Stern-Volmer equation. The quenching constants are increasing with growing pH. The decay of fluorescence is monoexponential with lifetime about 8.4 ns, which does not depend on pH and glucose concentration indicating static quenching. The quenching constant can be interpreted as apparent equilibrium constant of estrification of boronic group with diol. Quantum dots are also quenching 3-amino phenylboronic acid fluorescence. Fluorescence lifetime, in this case, is slightly decreasing with increasing concentration of quantum dots. The quenching constants are increasing slightly with pH's growth. Quenching mechanism of 3-amino phenylboronic acid fluorescence by quantum dots needs further experiments to be fully explained.
本文介绍了 3-氨基苯硼酸与葡萄糖或 ZnS:Cu 量子点相互作用的初步研究结果。采用巯基丙酸作为稳定剂合成并表征了 ZnS:Cu 量子点。通过稳态和时间分辨测量研究了 3-氨基苯硼酸荧光的猝灭。对于与葡萄糖的荧光猝灭，稳态测量结果符合 Stern-Volmer 方程。猝灭常数随 pH 值的增大而增大。荧光衰减呈单指数衰减，寿命约为 8.4 ns，这与 pH 值和葡萄糖浓度无关，表明为静态猝灭。猝灭常数可以解释为硼酸基团与二醇酯化的表观平衡常数。量子点也猝灭 3-氨基苯硼酸的荧光。在这种情况下，随着量子点浓度的增加，荧光寿命略有下降。猝灭常数随 pH 值的增大略有增加。量子点猝灭 3-氨基苯硼酸荧光的机制需要进一步实验来充分解释。
