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α-DNA.IX：干扰核糖核酸酶H介导的水解和逆转录需要α-异头寡脱氧核糖核苷酸与天然信使核糖核酸进行平行退火。

Alpha-DNA.IX: Parallel annealing of alpha-anomeric oligodeoxyribonucleotides to natural mRNA is required for interference in RNase H mediated hydrolysis and reverse transcription.

作者信息

Gagnor C, Rayner B, Leonetti J P, Imbach J L, Lebleu B

机构信息

Laboratoire de Biochimie des Protéines, UA 1191 CNRS, Université des Sciences et Techniques du Languedoc, Montpellier, France.

出版信息

Nucleic Acids Res. 1989 Jul 11;17(13):5107-14. doi: 10.1093/nar/17.13.5107.

DOI:10.1093/nar/17.13.5107
PMID:2474793
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318098/
Abstract

ps- and aps-alpha anomeric oligodeoxyribonucleotides were designed to recognize in parallel (ps) or antiparallel (aps) orientation two different sites of a 1000 base-long mRNA. Northern blots experiments indicate that only ps-alpha-oligonucleotides were able to hybridize to the mRNA target. Furthermore, only ps-alpha-oligonucleotides were able, in a sequence specific way, to protect the mRNA target against RNase H mediated hydrolysis or to inactivate the priming capacity of beta-oligodeoxynucleotide probes in reverse transcription. Formation of parallel-stranded mRNA alpha-oligonucleotide miniduplexes which prevents hybridization of beta-oligonucleotide probes is the most likely mechanism accounting for these results. Use of alpha-oligonucleotides as potential gene control agents is discussed.

摘要

设计了ps-α和aps-α异头寡脱氧核糖核苷酸，使其以平行（ps）或反平行（aps）方向识别1000个碱基长的mRNA的两个不同位点。Northern印迹实验表明，只有ps-α寡核苷酸能够与mRNA靶标杂交。此外，只有ps-α寡核苷酸能够以序列特异性方式保护mRNA靶标免受RNase H介导的水解，或使β-寡脱氧核苷酸探针在逆转录中的引发能力失活。防止β-寡核苷酸探针杂交的平行链mRNA-α寡核苷酸小双链体的形成是解释这些结果的最可能机制。讨论了将α-寡核苷酸用作潜在基因控制剂的用途。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b0/318098/0590d893d27e/nar00130-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b0/318098/8ad661abf44f/nar00130-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b0/318098/a5c5ae462796/nar00130-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b0/318098/0590d893d27e/nar00130-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b0/318098/8ad661abf44f/nar00130-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b0/318098/a5c5ae462796/nar00130-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b0/318098/0590d893d27e/nar00130-0201-a.jpg

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