α-DNA-III. 非天然十六脱氧核糖核苷酸α-[d(CpApTpGpCpG)]和α-[d(CpGpCpApTpG)]的高场1H-NMR表征、反平行自我识别及构象。α-寡脱氧核苷酸作为基因控制的潜在细胞探针。
alpha-DNA-III. Characterization by high field 1H-NMR, anti-parallel self-recognition and conformation of the unnatural hexadeoxyribonucleotides alpha-[d(CpApTpGpCpG)] and alpha-[d(CpGpCpApTpG)]. Alpha-oligodeoxynucleotides as potential cellular probes for gene control.
作者信息
Morvan F, Rayner B, Imbach J L, Chang D K, Lown J W
出版信息
Nucleic Acids Res. 1987 May 26;15(10):4241-55. doi: 10.1093/nar/15.10.4241.
Abstract
High field 2-D-1H-NMR techniques permitted the assignment of all non-exchangeable protons of the unnatural deoxyribonucleotides alpha-[d(CpApTpGpCpG)] and alpha-[d(CpGpCpApTpG)]. 1-D and 2-D NOESY experiments show strong H6H8-H4' dipolar interactions for all nucleotides in both sequences. These data, together with COSY and J-resolved spectra, indicate that these two alpha-oligomers adopt 3'-exo conformations of the sugar moieties in solution with anti conformations of the glycosyl linkages. Both 1H-NMR data, and hypochromocity comparison of alpha-CATGCG and beta-CATGCG, demonstrate a higher degree of base stacking in the case of the alpha-sequence. The UV hyperchromicity at 260 nm, and symmetry considerations in the imino proton NMR experiments reveal antiparallel self-recognition and duplex annealing at positions 1-4 for alpha-[d(CATGCG)] and positions 3-6 for alpha-[d(CGCATG)]. The temperature variation of the imino proton NMR signals suggests that the hydrogen bonding in self-recognition is comparable in strength with that in a beta-DNA duplex, and NOE data are in accord with Watson-Crick rather than Hoogsteen base pairing.
摘要
高场二维¹H-NMR技术能够确定非天然脱氧核糖核苷酸α-[d(CpApTpGpCpG)]和α-[d(CpGpCpApTpG)]的所有非交换质子。一维和二维NOESY实验表明，在这两个序列中，所有核苷酸的H6H8-H4'之间都存在强烈的偶极相互作用。这些数据与COSY和J-分解谱一起表明，这两种α-寡聚物在溶液中糖部分采取3'-外向构象，糖苷键为反式构象。¹H-NMR数据以及α-CATGCG和β-CATGCG的减色性比较表明，α-序列的碱基堆积程度更高。260 nm处的紫外增色性以及亚氨基质子NMR实验中的对称性考虑揭示了α-[d(CATGCG)]在1-4位以及α-[d(CGCATG)]在3-6位的反平行自我识别和双链退火。亚氨基质子NMR信号的温度变化表明，自我识别中的氢键强度与β-DNA双链中的相当，并且NOE数据符合沃森-克里克碱基配对而非霍格施泰因碱基配对。
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