School of Life Science and Technology, Dalian Ocean University, Dalian 116023, China.
Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Carbohydr Polym. 2012 Sep 1;90(1):491-5. doi: 10.1016/j.carbpol.2012.05.070. Epub 2012 Jun 2.
The interactions of tobacco cells with two oceanic oligosaccharides, chitosan oligosaccharide (COS) and alginate derived oligosaccharide (AOS) were investigated and compared by time-resolved fluorometric method using two Eu(3+) complexes as luminescence probes. The binding processes of two oligosaccharides and tobacco leaf cells (epidermal and stomatal guard cells) were characterized by the luminescence imaging method based on the reaction of tobacco cells with oligosaccharide (COS or AOS) conjugated to a highly fluorescent Eu(3+) complex. In addition, the concentration changes of indole-3-acetic acid (IAA) and IAA-related peroxidase produced in the cells during the interaction of oligosaccharides (COS or AOS) and tobacco cells were also determined. The results indicate that the bioactivity of COS, with faster binding rate to the tobacco cells and stronger effect on the IAA generation in the cells, is remarkably higher than that of AOS. The comparison study on bioactivities of the two oligosaccharides reveals the relationship between structures and bioactivities of oligosaccharide, and suggests that COS should be more favorable to be used as a promotion reagent of plant growth in agriculture field.
采用时间分辨荧光法，利用两种 Eu(3+) 配合物作为荧光探针，研究并比较了烟草细胞与两种海洋低聚糖（壳寡糖（COS）和藻酸盐衍生低聚糖（AOS））的相互作用。通过基于烟草细胞与寡糖（COS 或 AOS）与高荧光 Eu(3+) 配合物缀合的反应的荧光成像方法，表征了两种低聚糖与烟草叶片细胞（表皮和气孔保卫细胞）的结合过程。此外，还测定了在寡糖（COS 或 AOS）与烟草细胞相互作用过程中细胞内吲哚-3-乙酸（IAA）和 IAA 相关过氧化物酶的浓度变化。结果表明，COS 的生物活性更高，其与烟草细胞的结合速率更快，对细胞内 IAA 生成的影响更强，而 AOS 的生物活性则较低。对两种低聚糖生物活性的比较研究揭示了低聚糖结构与生物活性之间的关系，并表明 COS 更有利于在农业领域用作植物生长的促进剂。
