Stewart H J, McCann S H, Northrop A J, Lamming G E, Flint A P
AFRC Research Group on Hormones and Farm Animal Reproduction, Babraham, Cambridge.
J Mol Endocrinol. 1989 Jan;2(1):65-70. doi: 10.1677/jme.0.0020065.
A cloned cDNA has been isolated by probing a sheep blastocyst cDNA library using a synthetic oligonucleotide representing the N-terminal amino acid sequence of the antiluteolytic protein, ovine trophoblast protein-1. Sequence analysis of the cDNA confirms the 70% homology between the antiluteolysin and the interferon-alpha family of proteins; however, the sequence reported here differs at several points from previously reported amino acid and cDNA sequences for the antiluteolysin. In-vitro translation of day-16 poly(A)+ RNA indicated that antiluteolysin mRNA is a major constituent of total mRNA at this stage of blastocyst development, and Northern blotting confirmed that antiluteolysin mRNA production occurred between days 13 and 22 after oestrus. This is consistent with the stage at which embryonic extracts are antiluteolytic on administration in vivo. These and other data confirm that the ovine trophoblast antiluteolysin is an interferon, and suggest that at least five isoforms of this protein may exist.
通过使用代表抗黄体溶解蛋白（绵羊滋养层蛋白-1）N端氨基酸序列的合成寡核苷酸探测绵羊囊胚cDNA文库，已分离出一个克隆的cDNA。对该cDNA的序列分析证实了抗黄体溶解素与α-干扰素蛋白家族之间70%的同源性；然而，此处报道的序列在几个点上与先前报道的抗黄体溶解素的氨基酸和cDNA序列不同。第16天的多聚腺苷酸加尾RNA的体外翻译表明，抗黄体溶解素mRNA是囊胚发育这一阶段总mRNA的主要成分，Northern印迹法证实抗黄体溶解素mRNA在发情后第13至22天产生。这与胚胎提取物在体内给药时具有抗黄体溶解作用的阶段一致。这些及其他数据证实绵羊滋养层抗黄体溶解素是一种干扰素，并表明该蛋白可能至少存在五种同工型。
