从以废食用油为培养基生长的食酸戴尔福特菌细胞中回收无定形聚羟基丁酸酯颗粒。
Recovery of amorphous polyhydroxybutyrate granules from Cupriavidus necator cells grown on used cooking oil.
作者信息
Martino Lucrezia, Cruz Madalena V, Scoma Alberto, Freitas Filomena, Bertin Lorenzo, Scandola Mariastella, Reis Maria A M
机构信息
Department of Chemistry "G. Ciamician", Alma Mater Studiorum - University of Bologna, via Selmi 2, I-40126 Bologna, Italy.
REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
出版信息
Int J Biol Macromol. 2014 Nov;71:117-23. doi: 10.1016/j.ijbiomac.2014.04.016. Epub 2014 Apr 19.
Used cooking oil (UCO) was employed as the sole carbon source for the production of polyhydroxybutyrate (PHB) by cultivation in batch mode of Cupriavidus necator DSM 428. The produced biomass was used for extraction of the PHB granules with a solvent-free approach using sodium dodecyl sulfate (SDS), ethylenediaminetetraacetic acid (EDTA), and the enzyme Alcalase in an aqueous medium. The recovered PHB granules showed a degree of purity higher than 90% and no crystallization (i.e., granules were recovered in their 'native' amorphous state) as demonstrated by wide angle X-ray diffraction (WAXS). Granules were characterized according to their thermal properties and stability by differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). Results show that UCO can be used as a renewable resource to produce amorphous PHB granules with excellent properties in a biocompatible manner.
使用废弃食用油（UCO）作为唯一碳源，通过分批培养模式培养食酸铜绿假单胞菌DSM 428来生产聚羟基丁酸酯（PHB）。所产生的生物质用于在水性介质中采用无溶剂方法，使用十二烷基硫酸钠（SDS）、乙二胺四乙酸（EDTA）和碱性蛋白酶Alcalase提取PHB颗粒。如广角X射线衍射（WAXS）所示，回收的PHB颗粒纯度高于90%且无结晶现象（即颗粒以其“天然”无定形状态回收）。通过差示扫描量热法（DSC）和热重分析（TGA）对颗粒的热性能和稳定性进行了表征。结果表明，UCO可以作为一种可再生资源，以生物相容的方式生产具有优异性能的无定形PHB颗粒。
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