Wang Liyang, Cui Yanmei, Liao Wenting, Liu Side
Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Apr;34(4):546-51.
To investigate the role of Sam68 (Src-associated substrate during mitosis 68 kD) in the occurrence and development of colorectal cancer.
Colorectal cancer cell lines with stable Sam68 over-expressing and low Sam68 expression were established to test the effect of Sam68 in the proliferation, invasion, and migration of the cancer cells using colony formation, MTT and Transwell assays.
SW480 and Ls174t colorectal cell lines over-expressing Sam68 showed significantly enhanced cell proliferation, invasion and migration (P<0.05). Conversely, the low Sam68 expression in SW620 and HCT116 colorectal cell lines significantly suppressed the cell proliferation, invasion and migration (P<0.05).
The expression of Sam68 can promote the proliferation, invasion and migration of colorectal cancer cells lines in vitro.
探讨Sam68（有丝分裂68kD时Src相关底物）在结直肠癌发生发展中的作用。
建立稳定过表达Sam68和低表达Sam68的结直肠癌细胞系，采用集落形成、MTT和Transwell实验检测Sam68对癌细胞增殖、侵袭和迁移的影响。
过表达Sam68的SW480和Ls174t结直肠癌细胞系的细胞增殖、侵袭和迁移显著增强（P<0.05）。相反，SW620和HCT116结直肠癌细胞系中Sam68低表达显著抑制细胞增殖、侵袭和迁移（P<0.05）。
Sam68的表达可促进体外结直肠癌细胞系的增殖、侵袭和迁移。
