BMC Gastroenterol. 2013 Aug 9;13:126. doi: 10.1186/1471-230X-13-126.
Src-associated in mitosis (Sam68; 68 kDa) has been implicated in the oncogenesis and progression of several human cancers. The aim of this study was to investigate the clinicopathologic significance of Sam68 expression and its subcellular localization in colorectal cancer (CRC).
Sam68 expression was examined in CRC cell lines, nine matched CRC tissues and adjacent noncancerous tissues using reverse transcription (RT)-PCR, quantitative RT-PCR and Western blotting. Sam68 protein expression and localization were determined in 224 paraffin-embedded archived CRC samples using immunohistochemistry. Statistical analyses were applied to evaluate the clinicopathologic significance.
Sam68 was upregulated in CRC cell lines and CRC, as compared with normal tissues; high Sam68 expression was detected in 120/224 (53.6%) of the CRC tissues. High Sam68 expression correlated significantly with poor differentiation (P = 0.033), advanced T stage (P < 0.001), N stage (P = 0.023) and distant metastasis (P = 0.033). Sam68 nuclear localization correlated significantly with poor differentiation (P = 0.002) and T stage (P =0.021). Patients with high Sam68 expression or Sam68 nuclear localization had poorer overall survival than patients with low Sam68 expression or Sam68 cytoplasmic localization. Patients with high Sam68 expression had a higher risk of recurrence than those with low Sam68 expression.
Overexpression of Sam68 correlated highly with cancer progression and poor differentiation in CRC. High Sam68 expression and Sam68 nuclear localization were associated with poorer overall survival.
Src 相关在有丝分裂中（Sam68；68kDa）与几种人类癌症的发生和进展有关。本研究旨在探讨 Sam68 表达及其在结直肠癌（CRC）中的亚细胞定位的临床病理意义。
采用逆转录（RT）-PCR、实时定量 RT-PCR 和 Western blot 检测 CRC 细胞系、9 对 CRC 组织及其相邻非癌组织中 Sam68 的表达。采用免疫组织化学法检测 224 例石蜡包埋存档 CRC 样本中 Sam68 蛋白的表达和定位。应用统计学分析评估临床病理意义。
Sam68 在 CRC 细胞系和 CRC 中上调，与正常组织相比；在 224 例 CRC 组织中检测到 120/224（53.6%）高 Sam68 表达。高 Sam68 表达与分化不良显著相关（P=0.033），与高级 T 分期（P<0.001）、N 分期（P=0.023）和远处转移（P=0.033）显著相关。Sam68 核定位与分化不良显著相关（P=0.002）和 T 分期（P=0.021）。高 Sam68 表达或 Sam68 核定位患者的总生存期比低 Sam68 表达或 Sam68 细胞质定位患者差。高 Sam68 表达患者的复发风险高于低 Sam68 表达患者。
Sam68 在 CRC 中过表达与癌症进展和分化不良高度相关。高 Sam68 表达和 Sam68 核定位与总生存期较差相关。
