Zhang Rong-Kai, Yang Lu-Kun, Huang Li-Juan, Zhao Qing, Cai Dao-Zhang
Zhongguo Gu Shang. 2014 Jan;27(1):54-7.
To study the expression of lipoprotein related genes in subchondral bone of early experimental os-teoarthritis, which may play an important role in the pathogenesis of osteoarthritis.
Animals are equally divided into two groups: experimental group and control group, both of which contain fifteen rats of similar weight. The right knee joints of experimental group underwent surgery,which involved in both medial collateral ligament(MCL) transaction and medial meniscectomy, while the control group was only carried out with a sham operation. Rats were killed at 1, 2 and 4 weeks postsurgery to obtain the right knee joints. Total RNA of the subchondral bone was extracted,and then hybridized to Agilent Whole Rat Genome Microarray. Differentially expressed genes analysis was used to study the chemokine signaling pathway.
Apoa5 expression was down-regulated at 1, 2 weeks post-surgery, Apoc2 expression was up-regulated at 1 week after surgery, Apol3 expression was up-regulated at 1 and down-regulated at 4 weeks post-surgery, Lrp1 expression was down-regulated at 1, 2 weeks after surgery. Lrp5 was down-regulated at 2 weeks after surgery. Gpihbp1, Lpl, Tfpi and Vldlr were up-regulated at 1 weeks after surgery. Lrpap1 and RGD1309808 were down-regulated at 4 weeks after surgery.
Dysregulation of lipoprotein related genes plays an important role in pathogenesis of early experimental osteoarthritis.
研究早期实验性骨关节炎软骨下骨中脂蛋白相关基因的表达，这些基因可能在骨关节炎发病机制中起重要作用。
将动物平均分为两组：实验组和对照组，每组各有15只体重相近的大鼠。实验组大鼠右膝关节进行手术，包括切断内侧副韧带（MCL）和切除内侧半月板，而对照组仅进行假手术。术后1、2和4周处死大鼠以获取右膝关节。提取软骨下骨的总RNA，然后与安捷伦全大鼠基因组微阵列杂交。采用差异表达基因分析研究趋化因子信号通路。
术后1、2周Apoa5表达下调，术后1周Apoc2表达上调，术后1周Apol3表达上调，术后4周下调，术后1、2周Lrp1表达下调。术后2周Lrp5下调。术后1周Gpihbp1、Lpl、Tfpi和Vldlr上调。术后4周Lrpap1和RGD1309808下调。
脂蛋白相关基因失调在早期实验性骨关节炎发病机制中起重要作用。
