AgResearch Ltd, Grasslands Research Centre, Palmerston North, New Zealand.
FEMS Microbiol Ecol. 2014 Apr;88(1):94-106. doi: 10.1111/1574-6941.12273. Epub 2014 Jan 21.
Grasses of the tribe Triticeae were screened to determine the presence of mutualistic epichloae fungal endophytes. Over 1500 accessions, from more than 250 species, encompassing 22 genera within the Triticeae were screened using immunodetection and direct staining/microscopy techniques. Only two genera, Elymus and Hordeum, were identified as harbouring epichloae endophytes with accessions native to a range of countries including Canada, China, Iran, Kazakhstan, Kyrgyzstan, Mongolia, Russia and the USA. Genetic analysis based on simple sequence repeat data revealed that the majority of endophytes cluster according to geographical regions rather than to host species; many strains isolated from Hordeum grouped with those derived from Elymus, and amongst the Elymus-derived strains, there was no clear correspondence between clustering topology and host species. This is the first detailed survey demonstrating the genetic diversity of epichloae endophytes within the Triticeae and highlights the importance of germplasm centres for not only preserving the genetic diversity of plant species but also the beneficial microorganisms they may contain.
对小麦族植物进行了筛选，以确定是否存在互惠共生的内生真菌。使用免疫检测和直接染色/显微镜技术，对来自 250 多种、涵盖小麦族 22 个属的 1500 多个品系进行了筛选。结果仅发现两个属，即偃麦草属和大麦属，携带有内生真菌，这些内生真菌的宿主来自多个国家，包括加拿大、中国、伊朗、哈萨克斯坦、吉尔吉斯斯坦、蒙古、俄罗斯和美国。基于简单重复序列数据的遗传分析表明，大多数内生真菌根据地理区域而不是宿主物种聚类；从大麦中分离出的许多菌株与来自偃麦草的菌株聚在一起，而在源自偃麦草的菌株中，聚类拓扑结构与宿主物种之间没有明显的对应关系。这是首次详细调查，证明了内生真菌在小麦族中的遗传多样性，并强调了种质中心的重要性，不仅可以保存植物物种的遗传多样性，还可以保存它们可能含有的有益微生物。
