Laabich A, Sensenbrenner M, Delaunoy J P
Centre de Neurochimie du CNRS, Strasbourg, France.
Neurosci Lett. 1989 Aug 28;103(2):157-61. doi: 10.1016/0304-3940(89)90568-5.
We have studied the conditions to obtain ependymal cell cultures on porous bottom dishes and we succeeded to culture in a complete defined medium a continuous layer of primary ependymal cells from newborn rat cerebral hemispheres. This monolayer is composed of non-ciliated (35%) and ciliated ependymal cells (55%), with only a small contamination by astrocytes, oligodendrocytes and fibroblasts (10%). These cells grown on the microporous membrane are oriented and form a layer with an apical side and a basolateral side. We have demonstrated by using Trypan blue that between 14 and 24 days in culture the cells have formed a continuous monolayer. The presence of tight junctions between the cells has been shown by electron microscopy. Using immunocytochemical methods, we have studied the expression of glial fibrillary acidic protein (GFAP) and vimentin in these cultures.
我们研究了在多孔底部培养皿上获得室管膜细胞培养物的条件，并成功地在完全限定培养基中培养出了来自新生大鼠脑半球的连续的原代室管膜细胞层。该单层细胞由无纤毛的（35%）和有纤毛的室管膜细胞（55%）组成，仅有少量星形胶质细胞、少突胶质细胞和成纤维细胞污染（10%）。这些生长在微孔膜上的细胞呈定向排列，形成了具有顶端侧和基底外侧侧的一层细胞。我们通过台盼蓝染色证明，培养14至24天时细胞已形成连续的单层。电子显微镜显示了细胞间紧密连接的存在。我们使用免疫细胞化学方法研究了这些培养物中胶质纤维酸性蛋白（GFAP）和波形蛋白的表达。
