中国分离到的一种新型重配 H2N3 流感病毒。
A novel reassortant H2N3 influenza virus isolated from China.
机构信息
Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory of Surveillance and Early warning on Infectious Disease, Key Laboratory for Medical Virology, National Health and family planning Commission, Beijing 102206, China.
Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, Guangdong, China.
出版信息
Biomed Environ Sci. 2014 Apr;27(4):240-9. doi: 10.3967/bes2014.047.
OBJECTIVE
To analyze the genetic composition of a novel H2N3 virus isolate identified from a duck cage swab in a live poultry market (LPM) in 2009 in Guangdong province of China.
METHODS
PCR-positive specimens were inoculated into embryonated chicken eggs and subtyped by conventional RT-PCR. All segments of the virus A/environment/Guangdong/2/2009 were sequenced, and phylogenetic trees were constructed and analyzed.
RESULTS
The genes of this virus belong to Eurasian-lineage avian viruses. The virus is a reassortant with the HA gene from an H2N2 virus and the NA gene from an H5N3 virus. The PB1, PB2, and NP genes were from an H4N6 virus, the PA was from an H3N8 virus, the M gene was from an H1N3 virus, and the NS gene was from an H10N6 virus.
CONCLUSION
A novel avian-origin reassortant H2N3 influenza virus was detected in a live poultry market. Its potential impacts and evolution should be closely monitored.
目的
分析 2009 年在中国广东省一个活禽市场鸭笼拭子中发现的新型 H2N3 病毒分离株的遗传组成。
方法
用常规 RT-PCR 对 PCR 阳性标本进行接种和亚型分析。对病毒 A/环境/广东/2/2009 的所有片段进行测序，并构建和分析系统发育树。
结果
该病毒的基因属于欧亚禽病毒谱系。该病毒是一种重配病毒，其 HA 基因来自 H2N2 病毒，NA 基因来自 H5N3 病毒。PB1、PB2 和 NP 基因来自 H4N6 病毒，PA 基因来自 H3N8 病毒，M 基因来自 H1N3 病毒，NS 基因来自 H10N6 病毒。
结论
在活禽市场检测到一种新型的禽源重配 H2N3 流感病毒。应密切监测其潜在影响和进化。
Zotero 插件