Department of Emergency and ICU, Fourth Affiliated Hospital of Nanchang University, Nanchang 330003, Jiangxi, China.
School of Life Sciences and Food Engineering, Nanchang University, Nanchang 330031, Jiangxi, China.
Biomed Environ Sci. 2014 Apr;27(4):300-3. doi: 10.3967/bes2014.053.
This study aims to observe the protective effects of ginsenoside Rb1 on liver and lung in rats with septic shock and reveal its mechanism. Rats were randomly divided into three groups: sham, cecal ligation and puncture (CLP), and CLP with ginsenoside Rb1. Then, the survival rate, arterial blood pressure, TLR4 mRNA, and TNF-α levels were determined. The liver and lung tissues were stained with hematoxylin-eosin (HE). The overall survival rate of the Rb1 group was significantly higher than that of the CLP group. Mean arterial blood pressure went down in both the CLP and Rb1 groups after CLP, and there was a significant difference both in the sham and Rb1 groups when compared with the CLP group. The Rb1 treatment group had markedly lower TLR4 mRNA expression and TNF-α levels than the CLP group. In the CLP group, pathology showed swelling, degeneration, necrosis, and neutrophil infiltration in the liver and alveolar epithelial cells. However, in the Rb1 group, there was mild degeneration and slight neutrophil infiltration, but no obvious necrosis. Rb1 may improve the survival rate, ameliorate arterial blood pressure, and protect the liver and lung in septic shock rats by downregulating the expression of TLR4 mRNA and inhibiting the production of TNF-α.
本研究旨在观察人参皂苷 Rb1 对脓毒性休克大鼠肝肺的保护作用，并探讨其机制。将大鼠随机分为三组：假手术组、盲肠结扎穿孔（CLP）组和 CLP 加人参皂苷 Rb1 组。然后，测定大鼠的存活率、动脉血压、TLR4mRNA 和 TNF-α 水平。对肝肺组织进行苏木精-伊红（HE）染色。Rb1 组的总体存活率明显高于 CLP 组。CLP 后 CLP 组和 Rb1 组的平均动脉血压均下降，与 CLP 组相比，假手术组和 Rb1 组的平均动脉血压均有显著差异。Rb1 治疗组 TLR4mRNA 表达和 TNF-α 水平明显低于 CLP 组。在 CLP 组中，肝脏和肺泡上皮细胞出现肿胀、变性、坏死和中性粒细胞浸润；然而，在 Rb1 组中，仅观察到轻度变性和轻度中性粒细胞浸润，无明显坏死。Rb1 可能通过下调 TLR4mRNA 的表达和抑制 TNF-α 的产生，提高脓毒性休克大鼠的存活率，改善动脉血压，保护肝肺。
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