Jin Shuifang, Jiang Ronglin, Lei Shu, Jin Liming, Zhu Chen, Feng Wen, Shen Yingying
Intensive Care Unit, First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China.
Department of General Surgery, First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China.
Turk J Gastroenterol. 2019 Apr;30(4):364-371. doi: 10.5152/tjg.2019.18418.
BACKGROUND/AIMS: The aim of present study was to assess the protective effects of Shenfu injection (SI) on the intestinal mucosa and its regulation on the mucosal immune responses in rats with sepsis.
Sprague-Dawley rats were randomly divided into the sham, model, low-dose SI (LSF), and high-dose SI (HSF) groups. Sham animals underwent laparotomy only, whereas sepsis was modeled by cecal ligation and puncture in the remaining groups. At 2 h post-surgery, the LSF and HSF groups were intraperitoneally administered 5 and 20 mL/kg SI, respectively, whereas other animals with saline. At 12 h and 24 h post-surgery, eight rats per group were sacrificed, and blood and intestinal tissues were collected. The intestinal mucosa was analyzed by hematoxylin and eosin staining. Serum tumor necrosis factor (TNF)-α and interleukin (IL)-6 concentrations, as well as secretory immunoglobulin A (sIgA) content in the intestinal mucosa, were evaluated by enzyme-linked immunosorbent assay. CD3 and γδT lymphocytes were quantified by flow cytometry. Animal survival until 72 h was also recorded.
Intestinal mucosal injury was significantly higher in model animals than in sham animals at postoperative 12 h and 24 h. Serum TNF-α and IL-6 levels were markedly increased, whereas sIgA and CD3 and γδT cell amounts were overtly decreased (p<0.01). The LSF and HSF rats showed lower mortality, intestinal mucosal injury, and serum TNF-α and IL-6 levels (p<0.05), as well as higher sIgA levels and CD3 and γδT cell amounts, than the model group (p<0.01), with a dose-dependent manner.
SI dose-dependently prolongs survival and protects the intestinal mucosa in rats with sepsis, possibly through strengthening innate immunity instead of acquired immunity.
背景/目的：本研究旨在评估参附注射液（SI）对脓毒症大鼠肠黏膜的保护作用及其对黏膜免疫反应的调节作用。
将Sprague-Dawley大鼠随机分为假手术组、模型组、低剂量参附注射液组（LSF）和高剂量参附注射液组（HSF）。假手术组动物仅接受剖腹手术，而其余组通过盲肠结扎和穿刺建立脓毒症模型。术后2小时，LSF组和HSF组分别腹腔注射5和20 mL/kg的SI，而其他动物注射生理盐水。术后12小时和24小时，每组处死8只大鼠，收集血液和肠组织。通过苏木精-伊红染色分析肠黏膜。采用酶联免疫吸附测定法评估血清肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6浓度以及肠黏膜中分泌型免疫球蛋白A（sIgA）含量。通过流式细胞术对CD3和γδT淋巴细胞进行定量。记录动物至72小时的存活情况。
术后12小时和24小时，模型动物的肠黏膜损伤明显高于假手术组动物。血清TNF-α和IL-6水平显著升高，而sIgA以及CD3和γδT细胞数量明显减少（p<0.01）。与模型组相比，LSF组和HSF组大鼠的死亡率、肠黏膜损伤以及血清TNF-α和IL-6水平较低（p<0.05），sIgA水平以及CD3和γδT细胞数量较高（p<0.01），呈剂量依赖性。
参附注射液可能通过增强天然免疫而非获得性免疫，以剂量依赖方式延长脓毒症大鼠的存活时间并保护其肠黏膜。
