Wolfes H, Kogawa K, Millette C F, Cooper G M
Dana-Farber Cancer Institute, Boston, MA.
Science. 1989 Aug 18;245(4919):740-3. doi: 10.1126/science.2475907.
The expression of proto-oncogenes representative of several functional categories has been investigated during development of mouse male germ cells. The c-raf proto-oncogene and three members of the c-ras gene family were expressed in mitotically active stem cells, throughout the prophase of meiosis and to varying extents in post-meiotic cell types. In contrast, the nuclear proto-oncogenes c-fos, c-jun, and c-myc were specifically expressed at high levels in type B spermatogonia. High levels of c-myc and c-jun RNAs were also detected in spermatocytes early in the prophase of meiosis. The type B spermatogonia represent the last mitotic cell division before entry into meiotic prophase; therefore, these nuclear proto-oncogenes may be involved in altering programs of gene expression at this developmental transition.
在小鼠雄性生殖细胞发育过程中，对代表几个功能类别的原癌基因的表达进行了研究。原癌基因c-raf和c-ras基因家族的三个成员在有丝分裂活跃的干细胞中表达，在减数分裂前期全程表达，并在减数分裂后细胞类型中不同程度地表达。相比之下，核原癌基因c-fos、c-jun和c-myc在B型精原细胞中特异性高表达。在减数分裂前期早期的精母细胞中也检测到高水平的c-myc和c-jun RNA。B型精原细胞代表进入减数分裂前期之前的最后一次有丝分裂细胞分裂；因此，这些核原癌基因可能在这个发育转变过程中参与改变基因表达程序。
