Nováková Lucie, Perrenoud Alexandre Grand-Guillaume, Francois Isabelle, West Caroline, Lesellier Eric, Guillarme Davy
Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, Hradec Králové 500 05, Czech Republic.
School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, Geneva 4 1211, Switzerland.
Anal Chim Acta. 2014 May 8;824:18-35. doi: 10.1016/j.aca.2014.03.034. Epub 2014 Mar 28.
This tutorial provides an overview of the possibilities, limitations and analytical conditions of modern analytical supercritical fluid chromatography (SFC) using columns packed with sub-2 μm particles. In particular, it gives a detailed overview of commercially available modern SFC instrumentation and the detectors that can be employed (UV, MS, ELSD, FID, etc.). Some advice on the choice of the stationary phase dimensions and chemistries, the nature of the mobile phase (choice of organic modifier and additives) and its flow rate as well as the backpressure and temperature are also provided. Finally, several groups of potentially problematic compounds, including lipophilic compounds, hydrophilic substances and basic drugs, are discussed in detail. All these families of analytes can be resolved with SFC but require specific analytical conditions.
本教程概述了使用填充有亚 2 微米颗粒的色谱柱的现代分析超临界流体色谱（SFC）的可能性、局限性和分析条件。特别是，它详细介绍了市售的现代 SFC 仪器以及可使用的检测器（紫外、质谱、蒸发光散射检测器、氢火焰离子化检测器等）。还提供了一些关于固定相尺寸和化学性质、流动相性质（有机改性剂和添加剂的选择）及其流速以及背压和温度选择的建议。最后，详细讨论了几组潜在的疑难化合物，包括亲脂性化合物、亲水性物质和碱性药物。所有这些分析物家族都可以用 SFC 分离，但需要特定的分析条件。
