Si-Hung Le, Bamba Takeshi
Division of Metabolomics, Medical Institute of Bioregulation Kyushu University Fukuoka Japan.
Anal Sci Adv. 2021 Jan 21;2(1-2):47-67. doi: 10.1002/ansa.202000144. eCollection 2021 Feb.
The packed column supercritical fluid chromatography has risen as a promising alternative separation technique to the conventional liquid chromatography and gas chromatography. Although the packed column supercritical fluid chromatography has many advantages compared to other chromatographic techniques, its separation mechanism is not fully understood due to the complex combination effects of many chromatographic parameters on separation quality and the lacking of global strategies for studying separation mechanisms. This review aims to provide recent information regarding the chromatographic behaviors and the effects of the parameters on the separation, discuss the results, and point out the remaining bottlenecks in the packed column supercritical fluid chromatography retention mechanism studies.
填充柱超临界流体色谱法已成为一种有前途的替代分离技术，可替代传统的液相色谱法和气相色谱法。尽管与其他色谱技术相比，填充柱超临界流体色谱法有许多优点，但由于许多色谱参数对分离质量的复杂综合影响以及缺乏研究分离机制的整体策略，其分离机制尚未完全被理解。本综述旨在提供有关色谱行为以及参数对分离影响的最新信息，讨论结果，并指出填充柱超临界流体色谱保留机制研究中仍存在的瓶颈。
