[Change in nucleic acid synthesis in the spleen of rats with acute deficit of vitamin B1 during immunogenesis].

After four administrations of human blood serum gamma-globulin (intraperitoneally, once during 4 days at a dose of 1 mg and the following reimmunization within 60 days at a dose of 3-4 mg) distinct incorporation of 14C-thymidine (specific activity 54 muCi/mumole, by 5 muCi per an animal, 2 hrs before decapitation, intraperitoneally) into rat spleen DNA was detected within 4 days of immunogenesis. Acute oxythiamine B1 avitaminosis (single subcutaneous administration of oxythiamine at a dose of 400 mg/kg) led to a decrease in the label incorporation into spleen DNA of immunized rats by 70% (p less than 0.01) as compared with control immunized animals, while incorporation of 14C-thymidine was unaltered in intact rats. Content of RNA was not distinctly altered in spleen of B1-deficient rats within the 4 days of immunogenesis. Acute deficiency of vitamin B1 thus impaired synthesis of DNA in spleen during development of immunogenesis.