Sahori A, Katakami C, Kazusa R, Tetsumoto K, Fujisawa K, Yamamoto M
Nippon Ganka Gakkai Zasshi. 1989 Mar;93(3):375-83.
Epikeratophakia is a refractive surgery for the correction of aphakia, high myopia and keratoconus. Although many clinical studies of epikeratophakia have been performed, its wound healing process is not well understood. In the present study, we investigated keratocyte activities, particularly cell proliferation and collagen synthesis activity, during wound healing following epikeratophakia in rabbit corneas. Ten, 16, 28, 45, 63 and 90 days after the operation, corneas were excised, labeled with either 3H-thymidine (10 microCi/ml) or 3H-proline (10 microCi/ml) in DME for 4 hours and examined histologically, and by autoradiography. Ten days after the operation, almost no cells were seen in the keratolens except for a few keratocytes which had migrated to the edge of the keratolens. At days 16 and 28, keratocytes in the keratolens and in the host stroma near the junction between the host and the keratolens incorporated 3H-thymidine, suggesting active proliferation. The proliferating activity was no longer seen at days 45, 63 and 90. At day 90, almost complete repopulation with keratocytes was observed. Keratocytes in the keratolens and in the host stroma beneath the keratolens showed higher activity of 3H-proline incorporation than those in the control from day 16 through day 90 with the highest activity at day 28.
表层角膜镜片术是一种用于矫正无晶状体、高度近视和圆锥角膜的屈光手术。尽管已经进行了许多关于表层角膜镜片术的临床研究，但其伤口愈合过程仍未被充分了解。在本研究中，我们调查了兔眼角膜表层角膜镜片术后伤口愈合过程中的角膜细胞活性，特别是细胞增殖和胶原合成活性。术后10、16、28、45、63和90天，切除角膜，在含有3H-胸腺嘧啶核苷（10微居里/毫升）或3H-脯氨酸（10微居里/毫升）的杜氏改良伊格尔培养基中标记4小时，然后进行组织学检查和放射自显影。术后10天，除了一些迁移到角膜镜片边缘的角膜细胞外，在角膜镜片中几乎看不到细胞。在第16天和第28天，角膜镜片中和宿主与角膜镜片交界处附近的宿主基质中的角膜细胞掺入了3H-胸腺嘧啶核苷，表明有活跃的增殖。在第45、63和90天不再观察到增殖活性。在第90天，观察到角膜细胞几乎完全重新填充。从第16天到第90天，角膜镜片中和角膜镜片下方宿主基质中的角膜细胞显示出比对照组更高的3H-脯氨酸掺入活性，在第28天活性最高。
