Usuki Y, Katakami C, Yamamoto M
Department of Ophthalmology, Hyogo Prefectural Kakogawa Hospital, Japan.
Nippon Ganka Gakkai Zasshi. 1995 Nov;99(11):1209-13.
We investigated the effect of epidermal growth factor (EGF) on keratocytes during corneal wound healing focusing on cell proliferation. A penetrating linear incision was made in the center of rabbit corneas. The corneas were then treated with eye drops of recombinant human EGF (10 micrograms/ml) or physiological saline (control) four times a day. After 1, 2, 3, and 7 days, the corneas were excised, labeled with 3H-thymidine (10 microCi/ml) at 37 degrees C for 4 hours and subjected to autoradiography. The results demonstrated increased number of keratocytes incorporating 3H-thymidine on the 1st, 2nd, 3rd and 7th days of healing and accelerated stromal wound healing in corneas treated with EGF compared with the controls. Thus, EGF stimulates of proliferation keratocyte and promotes corneal stromal wound healing.
我们研究了表皮生长因子（EGF）在角膜伤口愈合过程中对角膜细胞的影响，重点关注细胞增殖。在兔角膜中央做一个穿透性线性切口。然后每天用重组人表皮生长因子滴眼液（10微克/毫升）或生理盐水（对照）滴眼4次。在第1、2、3和7天，切除角膜，在37℃下用3H-胸腺嘧啶核苷（10微居里/毫升）标记4小时，然后进行放射自显影。结果表明，与对照组相比，在愈合的第1、2、3和7天，摄取3H-胸腺嘧啶核苷的角膜细胞数量增加，且用EGF处理的角膜基质伤口愈合加快。因此，EGF刺激角膜细胞增殖并促进角膜基质伤口愈合。
