Zhou Weiqiang, Cai Menghao, Na Ke, Shen Chu, Zhang Xiaoxu, Zhou Xiangshan, Zhao Wenjie, Zhang Yuanxing
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
J Ind Microbiol Biotechnol. 2014 Jul;41(7):1169-73. doi: 10.1007/s10295-014-1445-0. Epub 2014 Apr 24.
The real-time distribution of anticancer 1403C in fermentation broth of marine fungus Halorosellinia sp. was investigated. It was closely related with pH variations, which was, 1403C in the supernatant decreased while that in the mycelia increased with pH rising. There was only 0.5 % of the total 1403C left in the supernatant when pH reached 7.0. The scanning electron microscope then provided information that compounds precipitated on the mycelia when pH rose. Then, the pH-regulation experiments proved that 1403C mainly secreted extracellular and easily dissolved in acidic condition but precipitated and absorbed on the mycelia with the increase of broth pH. Thereby, a pH-regulation strategy was proposed and applied to accumulate 1403C on the mycelia before draw-off of fermentation broth. It significantly simplified purification process and is critical for 1403C preparation of industrial scale.
研究了抗癌物质1403C在海洋真菌嗜盐黑孢霉发酵液中的实时分布情况。它与pH值变化密切相关，即随着pH值升高，上清液中的1403C减少，而菌丝体中的1403C增加。当pH值达到7.0时，上清液中仅残留总1403C的0.5%。扫描电子显微镜随后提供的信息表明，当pH值升高时，化合物会沉淀在菌丝体上。接着，pH调节实验证明，1403C主要分泌到细胞外，在酸性条件下易溶解，但随着发酵液pH值的升高会沉淀并吸附在菌丝体上。因此，提出了一种pH调节策略，并将其应用于在发酵液排出前在菌丝体上积累1403C。这显著简化了纯化过程，对工业规模制备1403C至关重要。
