Engineering Research Center of Groundwater Pollution Control and Remediation, Ministry of Education, College of Water Sciences, Beijing Normal University, Beijing, 100875, China,
Arch Environ Contam Toxicol. 2014 Jul;67(1):68-77. doi: 10.1007/s00244-014-0027-5. Epub 2014 Apr 24.
Thyroid hormone (TH) has long been known to be essential for normal brain development in both humans and animals, and increasing evidence suggests that environmental components may disrupt TH signaling. In the present study, two-hybrid yeast bioassay and chemical analysis were used to evaluate and identify thyroid-receptor (TR) disruptors in water from the Guanting Reservoir, Beijing, China. Modified yeast bioassay showed that the water samples could affect TH signaling. The bioassay-derived amiodarone hydrochloride equivalents ranged from 33.8 ± 3.3 to 308.5 ± 31.8 µg/L. Solid-phase extraction was used to separate the organic extracts, which were subjected to bioassay and chemical analysis. The organic extracts significantly antagonized the TR, which accounted for >86.0 % of the total effects. Thus, organic extracts may play a major role in the TR-disruption activity of the water. Phenols, organochlorine pesticides, and phthalate esters were detected in the organic extracts. Chemical analysis and toxic-equivalent calculation showed that a major cause of the TR antagonism of the water was dibutyl phthalate (80.1 to 122.7 %).
甲状腺激素 (TH) 早已被证实对人类和动物的正常大脑发育至关重要，越来越多的证据表明，环境成分可能会破坏 TH 信号。在本研究中，我们使用双杂交酵母生物测定法和化学分析来评估和识别来自中国北京官厅水库水中的甲状腺受体 (TR) 破坏剂。改良的酵母生物测定法表明，水样可能会影响 TH 信号。生物测定衍生的盐酸胺碘酮等效物的范围为 33.8±3.3 至 308.5±31.8µg/L。固相萃取用于分离有机提取物，然后对其进行生物测定和化学分析。有机提取物显著拮抗 TR，占总效应的>86.0%。因此，有机提取物可能在水中对 TR 破坏活性中起主要作用。在有机提取物中检测到酚类、有机氯农药和邻苯二甲酸酯。化学分析和毒性等效计算表明，水中 TR 拮抗作用的主要原因是邻苯二甲酸二丁酯（80.1 至 122.7%）。
