State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, People's Republic of China.
Environ Pollut. 2011 Feb;159(2):441-8. doi: 10.1016/j.envpol.2010.10.023. Epub 2010 Nov 12.
Thyroid hormone (TH) agonist and antagonist activities of water sources along the Yangtze River in China were surveyed by a green monkey kidney fibroblast (CV-1) cell-based TH reporter gene assay. Instrumental analysis was conducted to identify the responsible thyroid-active compounds. Instrumentally derived l-3,5,3'-triiodothyronine (T(3)) equivalents (T(3)-EQs) and thyroid receptor (TR) antagonist activity equivalents referring to dibutyl phthalate (DBP-EQs) were calculated from the concentrations of individual congeners. The reporter gene assay demonstrated that three out of eleven water sources contained TR agonist activity equivalents (TR-EQs), ranging from 286 to 293 ng T(3)/L. Anti-thyroid hormone activities were found in all water sources with the TR antagonist activity equivalents referring to DBP (Ant-TR-EQs), ranging from 51.5 to 555.3 μg/L. Comparisons of the equivalents from instrumental and biological assays suggested that high concentrations of DBP and di-2-ethylhexyl phthalate (DEHP) were responsible for the observed TR antagonist activities at some locations along the Yangtze River.
采用基于绿猴肾成纤维细胞（CV-1）的甲状腺激素报告基因检测法，调查了中国长江流域水源中的甲状腺激素（TH）激动剂和拮抗剂活性。采用仪器分析来鉴定具有甲状腺活性的化合物。根据个别同系物的浓度，从仪器衍生出 l-3,5,3'-三碘甲状腺原氨酸（T3）当量（T3-EQs）和二丁基邻苯二甲酸酯（DBP-EQs）甲状腺受体（TR）拮抗剂活性当量。报告基因检测法表明，11 个水源中有 3 个含有 TR 激动剂活性当量（TR-EQs），范围为 286 至 293ng T3/L。所有水源均表现出抗甲状腺激素活性，其 TR 拮抗剂活性当量参照二丁基邻苯二甲酸酯（Ant-TR-EQs），范围为 51.5 至 555.3μg/L。仪器和生物检测等效物的比较表明，在长江某些地段，DBP 和邻苯二甲酸二（2-乙基己基）酯（DEHP）的高浓度是观察到的 TR 拮抗剂活性的原因。
