Tian Xingui, Su Xiaobo, Xue Chunyan, Li Xiao, Zhou Zhichao, Zhou Rong
State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, 151 Yan Jiang Road, Guangzhou, 510120, China,
Virus Genes. 2014 Aug;49(1):22-9. doi: 10.1007/s11262-014-1071-y. Epub 2014 Apr 24.
Human adenovirus type 7 (HAdV-7) is one of the major serotypes responsible for acute respiratory infection. It is important to investigate the antigenic variabilities of different HAdV-7 genomic subtypes for vaccine development. Phylogenetic analysis of global HAdV-7 strains and major antigen proteins showed that HAdV-7 could be classified into two subtypes. There were three highly variable regions (HVR1, HVR4, and HVR7) in the hexon protein that varied between subtypes. Within each of the subtypes, these regions were conserved. Two subtype HAdV-7 strains isolated in China were used to immunize mice for antigenic characterization. Mice immunized with one subtype strain showed 4-8-fold lower neutralizing antibody titers against another subtype strain. ELISA results showed that the variation in HVR1, 4, and 7 regions contributed to antigenic change, and it may be concluded that the three regions contain subtype-specific epitopes. In summary, strains of HAdV-7 could be divided into two subtypes using genome sequence and antigenic analysis; our results could be important for HAdV-7 vaccine development.
人7型腺病毒（HAdV-7）是引起急性呼吸道感染的主要血清型之一。研究不同HAdV-7基因组亚型的抗原变异性对于疫苗开发很重要。对全球HAdV-7毒株和主要抗原蛋白进行系统发育分析表明，HAdV-7可分为两个亚型。六邻体蛋白中有三个高变区（HVR1、HVR4和HVR7），在不同亚型之间存在差异。在每个亚型内，这些区域是保守的。使用在中国分离的两株HAdV-7亚型毒株免疫小鼠以进行抗原特性分析。用其中一个亚型毒株免疫的小鼠对另一个亚型毒株的中和抗体效价低4至8倍。ELISA结果表明，HVR1、4和7区域的变异导致了抗原变化，可以得出结论，这三个区域包含亚型特异性表位。总之，利用基因组序列和抗原分析，HAdV-7毒株可分为两个亚型；我们的结果对于HAdV-7疫苗开发可能很重要。
