[结直肠癌组织中HIF-1α与Sema4D表达的相关性及临床意义]

[Correlation and clinical significance of expressions of HIF-1α and Sema4D in colorectal carcinoma tissues].

作者信息

Mu Linjun, Wang Jinshen, Guo Xiaobo, Zheng Sheng, Shan Keshu, Jing Changqing, Li Leping

机构信息

Department of Gastrointestinal Surgery, Provincial Hospital, Shandong University, Jinan 250021, China.

出版信息

Zhonghua Wei Chang Wai Ke Za Zhi. 2014 Apr;17(4):388-92.

PMID:24760652

Abstract

OBJECTIVE

To compare the expressions of hypoxia-inducible factor 1 alpha(HIF-1α) and Semaphorin 4D(Sema4D) in colorectal carcinoma and normal colorectal tissues, and to investigate their correlation and clinical significance.

METHODS

The expressions of HIF-1α and Sema4D were examined in 86 cases of colorectal carcinoma and 52 normal colorectal tissues by SP immunohistochemical staining. Correlation between these two expressions and association of the expressions with clinicopathological characters and prognosis were analyzed.

RESULTS

The positive rates of HIF-1α and Sema4D protein in colorectal carcinoma tissues were significantly higher than those in normal colorectal tissues(58.1% vs. 7.7%, χ(2)=34.624, P<0.01; 60.5% vs. 11.5%, χ(2)=31.839, P<0.01). HIF-1α and Sema4D protein expressions were closely associated with colorectal carcinoma histological types(P=0.003, P=0.010), TNM staging (P=0.003, P=0.017) and lymphatic metastasis (P=0.003, P=0.020), and a significant correlation was observed between the expressions of HIF-1α and Sema4D protein (r=0.567, P<0.01). The 5-year overall survival rate was 37%. Univariate analysis showed that 5-year survival rates of patients with positive and negative HIF-1α protein expression were 24% and 56%(P=0.003), and those with positive and negative Sema4D protein expression were 23% and 59%(P=0.001). Multivariate Cox analysis showed that expression of Sema4D was an independent prognostic factor of colorectal cancer patients(P=0.026), while expression of HIF-1α was not(P=0.501).

CONCLUSION

Combined detection of HIF-1α and Sema4D has the potential to predict the development trend of colorectal carcinoma and prognosis of patients.

摘要

目的

比较低氧诱导因子1α（HIF-1α）和信号素4D（Sema4D）在结直肠癌组织与正常结直肠组织中的表达情况，探讨二者的相关性及临床意义。

方法

采用SP免疫组化染色法检测86例结直肠癌组织及52例正常结直肠组织中HIF-1α和Sema4D的表达情况。分析二者表达之间的相关性以及它们与临床病理特征和预后的关系。

结果

结直肠癌组织中HIF-1α和Sema4D蛋白的阳性率显著高于正常结直肠组织（58.1%对7.7%，χ²=34.624，P<0.01；60.5%对11.5%，χ²=31.839，P<0.01）。HIF-1α和Sema4D蛋白表达与结直肠癌组织学类型（P=0.003，P=0.010）、TNM分期（P=0.003，P=0.017）及淋巴结转移（P=0.003，P=0.020）密切相关，且HIF-1α和Sema4D蛋白表达之间存在显著相关性（r=0.567，P<0.01）。5年总生存率为37%。单因素分析显示，HIF-1α蛋白表达阳性和阴性患者的5年生存率分别为24%和56%（P=0.003），Sema4D蛋白表达阳性和阴性患者的5年生存率分别为23%和59%（P=0.001）。多因素Cox分析显示，Sema4D表达是结直肠癌患者的独立预后因素（P=0.026），而HIF-1α表达不是（P=0.501）。