Department of Agricultural Microbiology, Tamilnadu Agricultural University, Coimbatore 641 003, Tamilnadu, India.
Int J Syst Evol Microbiol. 2014 Jul;64(Pt 7):2376-2384. doi: 10.1099/ijs.0.057232-0. Epub 2014 Apr 23.
Two strains of Gram-negative, methylotrophic bacteria, isolated because of their abilities to promote plant growth, were subjected to a polyphasic taxonomic study. The isolates were strictly aerobic, motile, pink-pigmented, facultatively methylotrophic, non-spore-forming rods. The chemotaxonomic characteristics of the isolates included the presence of C18 : 1ω7c as the major cellular fatty acid. The DNA G+C contents of strains BL36(T) and BL47(T) were 69.4 and 69.8 mol%, respectively. 16S rRNA gene sequence analysis of strains BL36(T) and BL47(T) placed them under the genus Methylobacterium, with the pairwise sequence similarity between them and the type strains of closely related species ranging from 97.2 to 99.0%. On the basis of their phenotypic and phylogenetic distinctiveness and the results of DNA-DNA hybridization analysis, the isolates represent two novel species within the genus Methylobacterium, for which the names Methylobacterium pseudosasicola sp. nov. (type strain BL36(T) = NBRC 105203(T) = ICMP 17621(T)) and Methylobacterium phyllostachyos sp. nov. (type strain BL47(T) = NBRC 105206(T) = ICMP 17619(T)) are proposed.
两株革兰氏阴性、甲基营养型细菌因具有促进植物生长的能力而被分离出来，对它们进行了多相分类学研究。这些分离物是严格需氧的、运动的、粉红色的、兼性甲基营养型的、非孢子形成的棒状细菌。分离物的化学生物分类特征包括存在 C18:1ω7c 作为主要细胞脂肪酸。菌株 BL36(T) 和 BL47(T) 的 DNA G+C 含量分别为 69.4 和 69.8 mol%。BL36(T)和 BL47(T)株的 16S rRNA 基因序列分析将它们置于甲基杆菌属中，与密切相关种的模式株之间的序列相似性在 97.2%到 99.0%之间。基于它们表型和系统发育的独特性以及 DNA-DNA 杂交分析的结果，这些分离物代表了甲基杆菌属中的两个新种，分别命名为 Methylobacterium pseudosasicola sp. nov.（模式株 BL36(T)=NBRC 105203(T)=ICMP 17621(T)）和 Methylobacterium phyllostachyos sp. nov.（模式株 BL47(T)=NBRC 105206(T)=ICMP 17619(T)）。
