Suppr超能文献

新种和新种,从土壤中分离得到。

sp. nov. and sp. nov., isolated from soil.

机构信息

Key Laboratory of Agricultural Microbiology of Heilongjiang Province, College of Plant Protection, Northeast Agricultural University, Harbin 150030, PR China.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.

出版信息

Int J Syst Evol Microbiol. 2024 Jul;74(7). doi: 10.1099/ijsem.0.006457.

Abstract

Two pink-pigmented bacteria, designated strains NEAU-140 and NEAU-K, were isolated from field soil collected from Linyi, Shandong Province, PR China. Both isolates were aerobic, Gram-stain-negative, rod-shaped, and facultatively methylotrophic. 16S rRNA gene sequences analysis showed that these two strains belong to the genus . Strain NEAU-140 exhibited high 16S rRNA gene sequence similarities to NBRC 15690 (97.43 %) and NBRC 105206 (97.36 %). Strain NEAU-K exhibited high 16S rRNA gene sequence similarities to NBRC 105206 (99.00 %) and DSM 23933 (98.72 %). A phylogenetic tree based on 16S rRNA gene sequences showed that strain NEAU-140 formed a clade with (95.94 %), (95.66 %) and (96.87 %), and strain NEAU-K formed a cluster with and . The predominant fatty acid in both strains was C 7. Both strains contained ubiquinone Q-10 as the only respiratory quinone. The polar lipid profiles of both strains contained diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. Whole-genome phylogeny showed that strains NEAU-140 and NEAU-K formed a phyletic line with , , , , , , and . The orthologous average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain NEAU-140 and its closely related strains were lower than 82.62 and 25.90  %, respectively. The ANI and dDDH values between strain NEAU-K and its closely related strains were lower than 86.29 and 31.7 %, respectively. The genomic DNA G+C contents were 71.63 mol% for strain NEAU-140 and 69.08 mol% for strain NEAU-K. On the basis of their phenotypic and phylogenetic distinctiveness and the results of dDDH and ANI hybridization, these two isolates represent two novel species within the genus , for which the names sp. nov. (type strain NEAU-140=MCCC 1K08801=DSM 110568) and sp. nov. (type strain NEAU-K=MCCC 1K08800=DSM 110567) are proposed.

摘要

两株粉红色着色细菌,分别命名为菌株 NEAU-140 和 NEAU-K,从中国山东省临沂市采集的田间土壤中分离得到。两株菌均为需氧、革兰氏阴性、杆状、兼性甲基营养型。16S rRNA 基因序列分析表明,这两株菌属于 属。菌株 NEAU-140 与 NBRC 15690(97.43%)和 NBRC 105206(97.36%)的 16S rRNA 基因序列相似度最高。菌株 NEAU-K 与 NBRC 105206(99.00%)和 DSM 23933(98.72%)的 16S rRNA 基因序列相似度最高。基于 16S rRNA 基因序列的系统发育树显示,菌株 NEAU-140 与 (95.94%)、 (95.66%)和 (96.87%)形成一个分支,菌株 NEAU-K 与 和 形成一个聚类。两株菌的主要脂肪酸均为 C7。两株菌均含有泛醌 Q-10 作为唯一的呼吸醌。两株菌的极性脂谱均含有双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱。全基因组系统发育分析表明,菌株 NEAU-140 和 NEAU-K 与 、 、 、 、 、 和 形成一个分支。与密切相关的菌株相比,菌株 NEAU-140 的正交平均核苷酸同一性(ANI)和数字 DNA-DNA 杂交(dDDH)值均低于 82.62%和 25.90%,菌株 NEAU-K 的 ANI 和 dDDH 值均低于 86.29%和 31.7%。菌株 NEAU-140 和 NEAU-K 的基因组 DNA G+C 含量分别为 71.63%mol 和 69.08%mol。基于表型和系统发育的独特性以及 dDDH 和 ANI 杂交的结果,这两个分离株代表了 属中的两个新种,分别命名为 sp. nov.(NEAU-140 型菌株=MCCC 1K08801=DSM 110568)和 sp. nov.(NEAU-K 型菌株=MCCC 1K08800=DSM 110567)。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验