Ren Jian-Xun, Li Lei, Lin Cheng-Ren, Fu Jian-Hua, Ma Yue-Ying, Li Jun-Mei, Li Hong-Hai, Wang Min, Liu Jian-Xun
Zhongguo Zhong Yao Za Zhi. 2014 Jan;39(2):285-90.
To observe the effect of formula of removing both phlegm and blood stasis (TYTZ) in inhibiting the inflammatory reaction in Chinese mini-swine with coronary atherosclerosis.
Totally 36 Chinese mini-swine were randomly divided to six groups: the normal control group, the model group, the Shujiangzhi group and TYTZ groups with does of 2.0, 1.0 and 0.5 g x kg(-1), and six each in every group. Except for the normal control group, all of other groups were fed with high-fat diet for 2 weeks. Interventional balloons are adopted to injure their left anterior descending artery endothelium. After the operation, they were fed with high-fat diet for 8 weeks to prepare the coronary atherosclerosis model. In the 8th week after the operation and administration, the intravascular ultrasound was adopted to observe the coronary artery plaque burden of each group and the pathological morphology of coronary artery. Such inflammatory factors as high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 were detected by ELISA. The expression of NF-kappaB p65 nuclear translocation was observed by the immunohistochemical method.
Compared with the normal control group, the model group showed significant increase in the coronary artery plaque burden at the end of the experiment (P < 0.01), notably abnormal structural changes in atherosclerotic vascular tissues, luminal stenosis, a large number of foam cells and inflammatory cell infiltration, remarkable growth of hs-CRP, TNF-alpha and IL-6 levels (P < 0.01). The immunohistochemical staining also showed the significant increase in the NF-kappaB p65 nuclear translocation of coronary artery of Chinese mini-swine in the model group. Compared with the model group, TYTZ could significantly attenuate atherosclerotic plaque burden (P < 0.01), inhibit the coronary luminal stenosis, reduce inflammatory cell infiltration, decrease such inflammatory cell factors as hs-CRP, TNF-alpha and IL-6 in serum, and inhibit the NF-kappaB p65 nuclear translocation of coronary artery (P < 0.05 or P < 0.01).
TYTZ can reduce the downstream inflammatory reaction by controlling NF-kappaB p65 nuclear translocation, so as to inhibit the occurrence and development of coronary atherosclerotic plaque in Chinese mini-swine.
观察祛痰化瘀方（TYTZ）对抑制中国小型猪冠状动脉粥样硬化炎症反应的作用。
将36只中国小型猪随机分为6组：正常对照组、模型组、舒降之组和剂量为2.0、1.0和0.5 g·kg⁻¹的TYTZ组，每组6只。除正常对照组外，其他各组均给予高脂饮食2周。采用介入球囊损伤其左前降支动脉内皮。术后给予高脂饮食8周以制备冠状动脉粥样硬化模型。在术后第8周给药后，采用血管内超声观察各组冠状动脉斑块负荷及冠状动脉病理形态。采用酶联免疫吸附测定法检测高敏C反应蛋白（hs-CRP）、肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6等炎症因子。采用免疫组织化学方法观察NF-κB p65核转位的表达。
与正常对照组相比，模型组实验结束时冠状动脉斑块负荷显著增加（P<0.01），动脉粥样硬化血管组织结构明显异常，管腔狭窄，大量泡沫细胞和炎症细胞浸润，hs-CRP、TNF-α和IL-6水平显著升高（P<0.01）。免疫组织化学染色也显示模型组中国小型猪冠状动脉NF-κB p65核转位显著增加。与模型组相比，TYTZ可显著减轻动脉粥样硬化斑块负荷（P<0.01），抑制冠状动脉管腔狭窄，减少炎症细胞浸润，降低血清中hs-CRP、TNF-α和IL-6等炎症细胞因子水平，并抑制冠状动脉NF-κB p65核转位（P<0.05或P<0.01）。
TYTZ可通过控制NF-κB p65核转位减少下游炎症反应，从而抑制中国小型猪冠状动脉粥样硬化斑块的发生发展。
