Business Unit Separation and Conversion Technology (SCT), Flemish Institute for Technological Research (VITO) , Boeretang 200, 2400 Mol, Belgium.
J Agric Food Chem. 2014 May 21;62(20):4812-31. doi: 10.1021/jf500285g. Epub 2014 May 6.
The aim of the present study was to develop a generic analytical method for the identification and quantitation of apolar plant metabolites in biomass using liquid chromatography-photodiode array-accurate mass mass spectrometry (LC-PDA-amMS). During this study, a single generic sample preparation protocol was applied to extract apolar plant metabolites. Compound identification was performed using a single generic screening method for apolar compounds without the need for dedicated fractionation. Such a generic approach renders vast amounts of information and is virtually limited by only the solubility and detector response of the metabolites of interest. Method validation confirmed that this approach is applicable for quantitative purposes. Furthermore, an identification-quantitation strategy based on amMS and molar extinction coefficients was used for carotenoids, eliminating the need for reference standards for each carotenoid. To challenge the validated method, chili peppers (Capsicum frutescens L.) were analyzed to unravel their complex phytochemical composition (carotenoids, glycolipids, glycerolipids, capsaicinoids, lipid-soluble vitamins).
本研究旨在开发一种通用的分析方法，用于使用液相色谱-光电二极管阵列-精确质量质谱（LC-PDA-amMS）鉴定和定量生物质中的非极性植物代谢物。在本研究中，采用单一的通用样品制备方案来提取非极性植物代谢物。化合物鉴定采用单一的非极性化合物通用筛选方法进行，无需专门的分级分离。这种通用方法可以提供大量信息，并且实际上仅受感兴趣的代谢物的溶解度和检测器响应的限制。方法验证证实，该方法适用于定量目的。此外，基于 amMS 和摩尔消光系数的鉴定-定量策略用于类胡萝卜素，从而消除了对每种类胡萝卜素的参考标准的需求。为了挑战验证方法，对辣椒（Capsicum frutescens L.）进行了分析，以揭示其复杂的植物化学成分（类胡萝卜素、糖脂、甘油酯、辣椒素、脂溶性维生素）。
