一种通过生物素-链霉亲和素系统扩增的用于检测非甾体抗炎药酮洛芬的灵敏酶联免疫吸附测定法。

A sensitive enzyme-linked immunosorbent assay amplified by biotin-streptavidin system for detecting non-steroidal anti-inflammatory drug ketoprofen.

作者信息

Bu Dan, Zhuang Hui S, Yang Guang X

机构信息

a Institute of Environmental Monitoring and Evaluation, School of Environment Science and Technology , Shanghai Jiao Tong University , Shanghai , China.

出版信息

J Environ Sci Health B. 2014;49(6):408-15. doi: 10.1080/03601234.2014.894776.

DOI:10.1080/03601234.2014.894776

PMID:24762178

Abstract

A sensitive biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) method was developed for detecting non-steroidal anti-inflammatory drug ketoprofen. Compared with traditional ELISA method, the sensitivity of proposed immunoassay was enhanced by the biotin-streptavidin system. Under the optimal condition, the median inhibitory concentration (IC50) was 0.25 ng mL(-1), with minor cross-reactivity to a number of structural analogs. This developed assay was successfully applied to detect the ketoprofen residues in different fish samples, and good recoveries (72.6-105.5%) were obtained. The results indicated that this immunoassay method could specifically detect trace ketoprofen residues and could be widely used for routine monitoring of food samples.

摘要

建立了一种灵敏的生物素-链霉亲和素放大酶联免疫吸附测定（BA-ELISA）方法用于检测非甾体抗炎药酮洛芬。与传统ELISA方法相比，所提出的免疫测定法的灵敏度通过生物素-链霉亲和素系统得到了提高。在最佳条件下，半数抑制浓度（IC50）为0.25 ng mL(-1)，对多种结构类似物的交叉反应较小。所建立的该测定法成功应用于检测不同鱼类样品中的酮洛芬残留，并获得了良好的回收率（72.6-105.5%）。结果表明，这种免疫测定方法能够特异性地检测痕量酮洛芬残留，可广泛用于食品样品的常规监测。