一种使用改良的生物素-链霉亲和素放大系统的敏感三明治 ELISA 法，用于检测鱼类、虾类和蟹类中的组胺。
A sensitive sandwich ELISA using a modified biotin-streptavidin amplified system for histamine detection in fish, prawn and crab.
机构信息
Centre of Molecular and Environmental Biology, University of Minho, Department of Biology, Campus de Gualtar, 4710-057 Braga, Portugal.
Centre of Molecular and Environmental Biology, University of Minho, Department of Biology, Campus de Gualtar, 4710-057 Braga, Portugal; Chinese-German Joint Institute for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China.
出版信息
Food Chem. 2021 Jul 15;350:129196. doi: 10.1016/j.foodchem.2021.129196. Epub 2021 Feb 5.
Histamine poisoning from seafood is a significant public health and safety concern. To detect histamine sensitively and accurately, a novel competitive sandwich immunoassay using a modified biotin-streptavidin system coupling with polylysine was developed. Using this strategy, a sandwich ELISA with an IC value of 112.8 ng mL and a broad linear range of 11.7-1500 ng mL with a correlation coefficient of 0.9942 was validated. Without any sample derivatization procedure, the recovery of histamine ranged from 80.19% to 108.3% with a coefficient of variation of 1.43-11.7% in tuna, prawn and crab. The sandwich ELISA had a detectionlimit of 5.86 ng mL, which was 15-fold lower than an indirect competitive ELISA (ic-ELISA). This simple, sensitive and accurate method can be applied to detect histamine in routine seafood samples.
海鲜引起的组胺中毒是一个重大的公共卫生和安全问题。为了灵敏准确地检测组胺，开发了一种使用改良的生物素-链霉亲和素系统与聚赖氨酸偶联的新型竞争 sandwich 免疫分析方法。利用该策略，建立了一种夹心 ELISA，IC 值为 112.8ng/mL，线性范围为 11.7-1500ng/mL，相关系数为 0.9942。无需任何样品衍生化程序，在金枪鱼、对虾和螃蟹中的组胺回收率在 80.19%至 108.3%之间，变异系数为 1.43-11.7%。夹心 ELISA 的检测限为 5.86ng/mL，比间接竞争 ELISA（ic-ELISA）低 15 倍。这种简单、灵敏和准确的方法可用于常规海鲜样品中组胺的检测。
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