School of Environment Science and Technology, Shanghai Jiao Tong University, No. 800, Dongchuan Road, Minghang District, Shanghai 200240, PR China.
School of Agriculture and Biology, Shanghai Jiao Tong University, No. 2678, Qixin Road, Minghang District, Shanghai 201101, PR China.
Talanta. 2014 Mar;120:40-6. doi: 10.1016/j.talanta.2013.11.080. Epub 2013 Dec 4.
Tetrabromobisphenol A (TBBPA) is a widely used brominated flame retardant. A sensitive and selective indirect competitive biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) was developed for detecting TBBPA. The optimal hapten of TBBPA was 2-(2,6-dibromo-4-(2-(3,5-dibromo-4-hydroxyphenly)propan-2-yl)) acetic acid. Several physiochemical factors that influence assay performance, such as optimal coupling concentration of immunogen and antibody, organic solvent, ionic strength, and pH, were studied and optimized. The limit of detection (IC10) was 0.027 ng/mL and the median inhibitory concentration (IC50) was 0.58 ng/mL. The BA-ELISA was highly selective, with low cross-reactivity with TBBPA analogs. Finally, the assay was used to detect TBBPA in electronic waste samples. The results are consistent with those using liquid chromatography, which proves that the proposed immunoassay is accurate and receptive. This BA-ELISA method is suitable for the rapid and sensitive screening of TBBPA in environmental monitoring.
四溴双酚 A（TBBPA）是一种广泛使用的溴化阻燃剂。本文建立了一种用于检测 TBBPA 的灵敏、选择性的间接竞争生物素-链霉亲和素放大酶联免疫吸附测定法（BA-ELISA）。TBBPA 的最佳半抗原为 2-(2,6-二溴-4-(2-(3,5-二溴-4-羟苯基)丙-2-基)）乙酸。研究并优化了影响测定性能的几种物理化学因素，如免疫原和抗体的最佳偶联浓度、有机溶剂、离子强度和 pH 值。检测限（IC10）为 0.027ng/mL，半数抑制浓度（IC50）为 0.58ng/mL。该 BA-ELISA 具有高度选择性，与 TBBPA 类似物的交叉反应性低。最后，该方法用于检测电子废物样品中的 TBBPA。结果与液相色谱法一致，证明了所提出的免疫测定法准确可靠。该 BA-ELISA 方法适用于环境监测中 TBBPA 的快速、灵敏筛选。
