Hugh A R, Simmons R D, Willenborg D O
Neurosciences Research Unit, Royal Canberra Hospital, Australia.
Cell Immunol. 1989 Oct 1;123(1):108-17. doi: 10.1016/0008-8749(89)90272-4.
When lymphoid cells from rats recovered from experimental autoimmune encephalomyelitis (EAE) were incubated in vitro for 1 hr with myelin basic protein (BP), then washed and transferred along with anti-BP immune serum to naive recipients, those recipients immediately developed a solid, long-lasting resistance to active induction of EAE. To obtain this high level of suppression, both steps of BP-incubation of cells and transfer of immune serum were found to be essential, i.e., direct transfer of nonincubated cells plus immune serum had no comparable suppressive effect, nor had transfer of BP-incubated cells with nonimmune serum. Specificity of the suppressive effect was indicated by the finding that cells from BP-sensitized donors, incubated with BP, protected against BP-CFA-induced disease but not against disease induced with whole spinal cord homogenate (SCH-CFA). As expected, cells from SCH-CFA-sensitized donors incubated with SCH protected recipients against disease induced with either SCH-CFA or BP-CFA. The suppression appears to act early in the afferent stage of the immune response, since inoculation with incubated cells as little as 24 hr after active challenge was ineffective. There was no suppression of passively induced disease.
将从实验性自身免疫性脑脊髓炎（EAE）恢复的大鼠的淋巴细胞，与髓鞘碱性蛋白（BP）在体外孵育1小时，然后洗涤，并与抗BP免疫血清一起转移给未接触过该疾病的受体，这些受体立即对EAE的主动诱导产生了稳固、持久的抵抗力。为了获得这种高水平的抑制作用，发现细胞与BP孵育以及免疫血清转移这两个步骤都是必不可少的，即直接转移未孵育的细胞加免疫血清没有可比的抑制作用，用非免疫血清转移经BP孵育的细胞也没有这种作用。抑制作用的特异性体现在以下发现中：来自BP致敏供体的细胞，与BP孵育后，可预防BP - 完全弗氏佐剂（CFA）诱导的疾病，但不能预防全脊髓匀浆（SCH - CFA）诱导的疾病。正如预期的那样，来自SCH - CFA致敏供体的细胞与SCH孵育后，可保护受体预防由SCH - CFA或BP - CFA诱导的疾病。这种抑制作用似乎在免疫反应的传入阶段早期起作用，因为在主动攻击后仅24小时接种经孵育的细胞是无效的。对被动诱导的疾病没有抑制作用。
