Driscoll B F, Kies M W, Alvord E C
J Immunol. 1982 Feb;128(2):635-8.
Experimental allergic encephalomyelitis (EAE) in strain 13 guinea pigs is an acute monophasic disease induced with myelin basic protein (BP) in complete Freund's adjuvant (CFA). We report here that EAE can be effectively suppressed by transfer of a limited number of EAE-effector cells. Lymph node cells (LNC) from donors sensitized with BP/CFA are activated in vitro by incubation with BP and strain 2 peritoneal exudate cells (PEC). 5 X 10(7) of these cells are capable of inducing lethal EAE in recipients; 0.5 to 1 X 10(7) cells (a suboptimal transfer) effectively suppress EAE induction in animals subsequently sensitized with BP/CFA. Suppressed recipients develop an early mild disease from which they recover completely. In contrast, suboptimal transfer of BP-sensitized cells does not protect recipients against sensitization with whole central nervous system (CNS) tissue/CFA. About 50% of these recipients succumb to acute EAE; the survivors develop chronic EAE of varying intensity: a mild chronic form with recovery, a severe chronic form that is eventually fatal, and a chronic relapsing form that results in permanent neurologic deficit. Preliminary attempts at detecting suppressor cells in the suppressed animals were unsuccessful. Instead, PEC of all recipients of suboptimal transfers were capable of transferring EAE to naive animals.
13 品系豚鼠的实验性变应性脑脊髓炎（EAE）是一种用髓鞘碱性蛋白（BP）加完全弗氏佐剂（CFA）诱发的急性单相疾病。我们在此报告，通过转移有限数量的 EAE 效应细胞可有效抑制 EAE。用 BP/CFA 致敏的供体的淋巴结细胞（LNC）在体外与 BP 和 2 品系腹腔渗出细胞（PEC）一起孵育后被激活。这些细胞中的 5×10⁷ 个能够在受体中诱发致死性 EAE；0.5 至 1×10⁷ 个细胞（次优转移）可有效抑制随后用 BP/CFA 致敏的动物的 EAE 诱导。受抑制的受体出现早期轻度疾病并完全康复。相比之下，次优转移 BP 致敏细胞不能保护受体免受全中枢神经系统（CNS）组织/CFA 致敏。这些受体中约 50%死于急性 EAE；幸存者发展为不同强度的慢性 EAE：一种恢复的轻度慢性形式、一种最终致命的重度慢性形式以及一种导致永久性神经功能缺损的慢性复发形式。在受抑制动物中检测抑制细胞的初步尝试未成功。相反，次优转移的所有受体的 PEC 都能够将 EAE 转移给未致敏的动物。
