Rellahan B L, Cone R E
Department of Pathology, University of Connecticut Health Center, Farmington 06032.
Cell Immunol. 1989 Oct 1;123(1):166-76. doi: 10.1016/0008-8749(89)90277-3.
Heterologous antisera which recognize non-major histocompatibility complex (MHC)-restricted T cell antigen-binding molecules (TABM) were used to characterize the expression and structure of TABM on thymic lymphocytes. Approximately 70% of thymocytes express membrane molecules bound by anti-TABM antibodies (mTABM). Antibody activity for thymocyte TABM could be removed by adsorption to splenic T cells, but not by adsorption to splenic B cells. Similarly, adsorption of the antiserum to thymocytes or splenic T cells removed antibody activity to a purified TABM whereas adsorption with B cells had no effect. Radioiodinated thymic and splenic T cell mTABM were resolved by 2D-polyacrylamide gel electrophoresis and when reduced, both populations of mTABM migrated primarily as Mr 23,000 proteins with an isoelectric point range of 6.8-7.8. Multimers of this protein were also observed at Mr 85-97,000 and 130-150,000 on both thymocytes and splenic T cells. These data indicate that MHC-unrestricted antigen-binding molecules are expressed by a majority of thymocytes and these thymic TABM are structurally and antigenically similar to mTABM on peripheral cells. This suggests an ontogenic relationship between thymic TABM and peripheral TABM.
利用识别非主要组织相容性复合体（MHC）限制性T细胞抗原结合分子（TABM）的异种抗血清来表征胸腺淋巴细胞上TABM的表达和结构。约70%的胸腺细胞表达与抗TABM抗体（mTABM）结合的膜分子。针对胸腺细胞TABM的抗体活性可通过吸附到脾T细胞而去除，但吸附到脾B细胞则不能去除。同样，抗血清吸附到胸腺细胞或脾T细胞可去除针对纯化TABM的抗体活性，而用B细胞吸附则无影响。通过二维聚丙烯酰胺凝胶电泳分离放射性碘化的胸腺和脾T细胞mTABM，还原后，两种mTABM群体主要以分子量23,000的蛋白质迁移，等电点范围为6.8 - 7.8。在胸腺细胞和脾T细胞上还观察到该蛋白质的多聚体，分子量分别为85 - 97,000和130 - 150,000。这些数据表明，大多数胸腺细胞表达MHC非限制性抗原结合分子，且这些胸腺TABM在结构和抗原性上与外周细胞上的mTABM相似。这提示胸腺TABM与外周TABM之间存在个体发生学关系。
