Redard M, Vassilakos P, Weintraub J
Department of Public Health, Canton of Geneva, Switzerland.
Diagn Cytopathol. 1989;5(2):188-93. doi: 10.1002/dc.2840050214.
We report a modified method to prepare cytologic specimens for the evaluation of estrogen receptors by immunocytochemistry. Cytologic samples were obtained by needle aspiration of tumor masses (nine patients) and body cavity fluids (nine patients). The cytologic material was divided, and slides for immunostaining were prepared according to two different techniques: (1) fixation of cells in suspension (modified method); and (2) fixation of cells adherent to slides (standard method). The results after immunostaining demonstrated that the number of cells adhering to slides prepared according to the modified method was greatly increased as compared with the number of cells remaining attached to slides prepared according to the standard method. There was no loss of cell groups or aggregates using the modified method. The antigenicity of estrophilin and the cell morphology were well-preserved for up to 30 days. With this modified method, cytologic samples may be stored and conveniently processed in batches, thereby improving the utilization of laboratory resources.
我们报告一种改良方法，用于制备通过免疫细胞化学评估雌激素受体的细胞学标本。通过针吸肿瘤肿块（9例患者）和体腔液（9例患者）获取细胞学样本。将细胞学材料分开，根据两种不同技术制备用于免疫染色的玻片：（1）悬浮细胞固定（改良方法）；（2）附着于玻片的细胞固定（标准方法）。免疫染色后的结果表明，与按照标准方法制备的玻片上残留的细胞数量相比，按照改良方法制备的玻片上附着的细胞数量大大增加。使用改良方法时细胞团或聚集体没有损失。雌激素受体的抗原性和细胞形态在长达30天内都得到了很好的保存。通过这种改良方法，细胞学样本可以储存并方便地批量处理，从而提高实验室资源的利用率。
