Brown P W, Witorsch R J, Banks L W, Lawrence W
Arch Surg. 1977 Feb;112(2):183-5. doi: 10.1001/archsurg.1977.01370020077008.
Sixteen patients underwent biopsy of primary or metastatic breast cancer and had equal portions of the tumors rapidly frozen by either the use of liquid nitrogen or the fluorinated hydrocarbon spray, Cryokwik. The estrogen receptor protein (ERP) assay was then performed on both specimens, utilizing the surcose gradient density method. In general, both methods of freezing gave similar results. Three patients had ERP measured after approximately three to four months of storage at -70 C. After a minimum of 14 weeks' storage, there was no statistically significant loss of activity by either method of preparation. The Cryokwik preparation method makes the accurate measurement of ERP possible for all community hospitals. The tissue may be prepared and stored until a convenient time for transportation to the appropriate laboratory.
16例原发性或转移性乳腺癌患者接受了活检，并将肿瘤的等份部分通过液氮或氟碳化合物喷雾（Cryokwik）迅速冷冻。然后使用蔗糖梯度密度法对两个标本进行雌激素受体蛋白（ERP）检测。一般来说，两种冷冻方法得到的结果相似。3例患者在-70℃储存约三到四个月后检测了ERP。在至少储存14周后，两种制备方法的活性均无统计学意义上的损失。Cryokwik制备方法使所有社区医院都能准确检测ERP。组织可以制备并储存，直到方便运送到合适的实验室。
