Pettinato G, Swanson P E, Insabato L, De Chiara A, Wick M R
Department of Pathology, Second Medical School, University of Naples, Italy.
Diagn Cytopathol. 1989;5(2):194-9. doi: 10.1002/dc.2840050215.
The recognition of myogenic differentiation is not always possible using routine light or electron microscopic techniques. In this article, we describe our experience with two small round-cell neoplasms occurring in young children, each of which exhibited an undifferentiated light microscopic appearance. In both cases, myogenic features were revealed by immunocytochemical methods applied to fine-needle aspiration (FNA) biopsies. Each was immunoreactive for desmin and vimentin and failed to react with antibodies to leukocyte-common antigen. Moreover, formalin-fixed tissue sections of one case were negative for cytokeratin and epithelial membrane antigen. Sporadic reactivity for neuron-specific enolase and Leu-7 antigen was observed in occasional cells in FNA specimens, but synaptophysin was not identified. The rapidity of the method and the reliability of the immunocytochemical findings, when compared with routine cytologic evaluation, emphasize the diagnostic utility of immunocytochemical analysis when FNAs of pediatric soft-tissue tumors are obtained.
使用常规光学或电子显微镜技术并不总是能够识别肌源性分化。在本文中，我们描述了我们对两名幼儿发生的两种小圆形细胞肿瘤的经验，每种肿瘤在光学显微镜下均表现为未分化外观。在这两个病例中，通过应用于细针穿刺（FNA）活检的免疫细胞化学方法揭示了肌源性特征。两者对结蛋白和波形蛋白均呈免疫反应性，而与白细胞共同抗原抗体无反应。此外，其中一例福尔马林固定组织切片的细胞角蛋白和上皮膜抗原呈阴性。在FNA标本中偶尔可见细胞对神经元特异性烯醇化酶和Leu-7抗原呈散在反应性，但未发现突触素。与常规细胞学评估相比，该方法的快速性和免疫细胞化学结果的可靠性强调了在获取儿科软组织肿瘤的FNA时免疫细胞化学分析的诊断效用。
