Wang Wei, Chen Nan-Yin, Zhang Wei, He Jun-Jun, Hang Zhe-Dong, Qin Fei, Dong Li-Na, Zhu Fa-Ming, Lyu Hang-Jun
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014 Apr;22(2):513-6. doi: 10.7534/j.issn.1009-2137.2014.02.045.
The aim of this study was to investigate the relatively frequencies of alleles in the HLA-C04:01:01G group and to analyze their relations with HLA-A and -B loci. DNA samples previously typed as HLA-C04:01:01G were sequentially selected. The sequences for exon 2 to 7 of the HLA-C locus were analyzed by polymerase chain reaction sequence-based typing(PCR-SBT). The HLA-A, -B, -DRB1 and -DQB1 loci were genotyped using PCR-SBT method. The results showed that 178 samples (94.2%) and 11 samples (5.8%) were assigned as HLA-C04:01:01 and HLA-C04:82 respectively among 189 samples previously typed as HLA-C04:01:01G. 72 haplotypes associated with HLA-C04:01:01 and C04:82 were found, in which the frequencies of 26 haplotypes were over 0.0050. HLA-C04:01:01 was strongly related with A02:03, A02:07, A11:01, A33:03, B13:01, B15:01, B15:05, B15:27, B40:01, B54:01 alleles, while HLA-C04:82 was related with B40:01. It is concluded that HLA-C04:01:01 and HLA-C04:82 alleles were confirmed in the HLA-C*04:01:01G group, which should be discriminated by the routine HLA genotyping.
