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通过聚合酶链反应序列分型法检测HLA - C基因座外显子1至7中的序列来鉴别HLA - C*07:01:01G和HLA - C*07:02:01G组中的等位基因

[Discrimination of alleles in HLA-C*07:01:01G and HLA-C*07:02:01G groups through detection sequences in exons 1 to 7 of HLA-C locus by using polymerase chain reaction sequence-based typing].

作者信息

Lü Hang-Jun, Zhang Wei, He Jun-Jun, He Yan-Min, Wang Wei, Hang Zhe-Dong, Chen Nan-Yin, Zhu Fa-Ming, Yan Li-Xing

机构信息

Institute of Transfusion Medicine, Blood Center of Zhejiang Province, Hangzhou, Zhejiang Province, China.

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Feb;20(1):178-81.

PMID:22391192
Abstract

This study was aimed to discriminate the alleles in the HLA-C07:01:01G and HLA-C07:02:01G groups and analyze their associations with HLA-B locus. Samples previously typed as HLA-C07:01:01G and HLA-C07:02:01G were collected. The nucleotide sequences in exons 1 to 7 of the HLA-C locus were sequenced by polymerase chain reaction sequence-based typing (PCR-SBT) and HLA-B genotyping was also preformed by PCR-SBT in these samples. The results showed that 4 samples (30.8%) were confirmed as HLA-C07:01:01 and 9 samples (69.2%) were HLA-C07:06 among 13 samples previously typed as HLA-C07:01:01G. Linkage disequilibrium (LD) analysis showed that HLA-C07:06 allele was strongly related with HLA-B44:03. All samples were typed as C07:02:01 among 102 individuals previously typed as C07:02:01G. LD analysis showed that C07:02:01 was strongly related with HLA-B51:01, B46:01, B39:01, B40:01, B38:02, B15:02 alleles. It is concluded that HLA-C07:01:01 and HLA-C07:06 alleles are confirmed in the HLA-C07:01:01G group and HLA-C07:02:01 is a preferred allele in the HLA-C*07:02:01G.

摘要

本研究旨在鉴别HLA - C07:01:01G和HLA - C07:02:01G组中的等位基因，并分析它们与HLA - B位点的关联。收集了先前分型为HLA - C07:01:01G和HLA - C07:02:01G的样本。通过基于聚合酶链反应序列分型（PCR - SBT）对HLA - C位点外显子1至7的核苷酸序列进行测序，同时也通过PCR - SBT对这些样本进行HLA - B基因分型。结果显示，在先前分型为HLA - C07:01:01G的13个样本中，4个样本（30.8%）被确认为HLA - C07:01:01，9个样本（69.2%）为HLA - C07:06。连锁不平衡（LD）分析表明，HLA - C07:06等位基因与HLA - B44:03密切相关。在先前分型为C07:02:01G的102名个体中，所有样本均分型为C07:02:01。LD分析表明，C07:02:01与HLA - B51:01、B46:01、B39:01、B40:01、B38:02、B15:02等位基因密切相关。结论是，在HLA - C07:01:01G组中确认了HLA - C07:01:01和HLA - C07:06等位基因，而HLA - C07:02:01是HLA - C*07:02:01G中的优势等位基因。

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