[Discrimination of alleles in HLA-C*07:01:01G and HLA-C*07:02:01G groups through detection sequences in exons 1 to 7 of HLA-C locus by using polymerase chain reaction sequence-based typing].

This study was aimed to discriminate the alleles in the HLA-C07:01:01G and HLA-C07:02:01G groups and analyze their associations with HLA-B locus. Samples previously typed as HLA-C07:01:01G and HLA-C07:02:01G were collected. The nucleotide sequences in exons 1 to 7 of the HLA-C locus were sequenced by polymerase chain reaction sequence-based typing (PCR-SBT) and HLA-B genotyping was also preformed by PCR-SBT in these samples. The results showed that 4 samples (30.8%) were confirmed as HLA-C07:01:01 and 9 samples (69.2%) were HLA-C07:06 among 13 samples previously typed as HLA-C07:01:01G. Linkage disequilibrium (LD) analysis showed that HLA-C07:06 allele was strongly related with HLA-B44:03. All samples were typed as C07:02:01 among 102 individuals previously typed as C07:02:01G. LD analysis showed that C07:02:01 was strongly related with HLA-B51:01, B46:01, B39:01, B40:01, B38:02, B15:02 alleles. It is concluded that HLA-C07:01:01 and HLA-C07:06 alleles are confirmed in the HLA-C07:01:01G group and HLA-C07:02:01 is a preferred allele in the HLA-C*07:02:01G.