Yin W-Z, Li F, Zhang L, Ren X-P, Zhang N, Wen J-F
Department of Gastroenterology, the Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China.
Eur Rev Med Pharmacol Sci. 2014;18(7):1027-32.
Increasing evidence indicates that MicroRNAs, a class of small RNA molecules, play crucial roles in tumorigenesis, through affecting cell proliferation, apoptosis and metastasis. The present study aimed to investigate the effect of miR-205 on gastric cancer cell proliferation.
The expression of miR-205 was examined in the gastric cancer tissues and cell lines. BrdU incorporation assay was used to measure the cell proliferation. Western blot was performed to determine the protein expression.
miR-205 is significantly down-regulated in gastric cancer tissues, compared with adjacent normal tissues. Besides, miR-205 expression is associated with clinical and pathological characteristics of patients. In vitro studies further found that inhibition of miR-205 significantly promoted gastric cancer cell proliferation via cell-cycle progression. Further analyses indicated that miR-205 was able to repress oncoprotein Yin Yang 1 expression, through targeting its 3'-untranlated region.
Our data suggest that down-regulation of miR-205 may represent an important mechanism for the development of gastric cancer.
越来越多的证据表明，微小RNA（一类小RNA分子）通过影响细胞增殖、凋亡和转移，在肿瘤发生过程中发挥关键作用。本研究旨在探讨miR-205对胃癌细胞增殖的影响。
检测胃癌组织和细胞系中miR-205的表达。采用BrdU掺入法检测细胞增殖。进行蛋白质印迹法测定蛋白质表达。
与邻近正常组织相比，miR-205在胃癌组织中显著下调。此外，miR-205的表达与患者的临床和病理特征相关。体外研究进一步发现，抑制miR-205可通过细胞周期进程显著促进胃癌细胞增殖。进一步分析表明，miR-205能够通过靶向阴阳1蛋白的3'-非翻译区来抑制其表达。
我们的数据表明，miR-205的下调可能是胃癌发生发展的重要机制。
