丙泊酚通过调控miR-205/YAP1轴抑制胃癌细胞的增殖和侵袭。
Propofol Inhibits Proliferation and Invasion of Stomach Cancer Cells by Regulating miR-205/YAP1 Axis.
作者信息
Xian Xiang-Shu, Wang Yu-Tie, Jiang Xiao-Meng
机构信息
Department of Gastroenterology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Qingdao 264000, People's Republic of China.
Department of Rheumatology and Immunology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Qingdao 264000, People's Republic of China.
出版信息
Cancer Manag Res. 2020 Oct 29;12:10771-10779. doi: 10.2147/CMAR.S270344. eCollection 2020.
BACKGROUND
Propofol is a common clinical intravenous anesthetic. In the last few years, studies have revealed that propofol not only has good anesthetic effect but also has certain anticancer effect. However, its role in stomach cancer (SC) and related mechanisms are still under investigation.
OBJECTIVE
This study was designed to determine the effect of propofol on SC and its related mechanisms.
METHODS
Purchased SC cells were treated with propofol at different concentrations (5, 10, and 20 μg/mL), miR-205 overexpression, and YAP1 inhibition. Then, the Cell Counting Kit-8 (CCK8), Transwell, and flow cytometry were carried out to determine the biological behavior changes of treated cells and the expression of miR-205 and YAP1 after treatment.
RESULTS
Propofol (10 μg/mL and 20 μg/mL) inhibited the growth of SC cells and promoted their apoptosis, and overexpressing miR-205 or inhibiting YAP1 can exert the same effects. In addition, propofol (10μg/mL and 20μg/mL) up-regulated miR-205 in SC cells. The dual-luciferase reporter assay revealed that YAP1 could be targeted and regulated by miR-205, and the rescue assay revealed that inhibiting miR-205 or overexpressing YAP1 could weaken the effect of propofol on the biological behaviors of SC cells.
CONCLUSION
Propofol can strongly suppress the proliferation and invasion of SC cells and induce their apoptosis via the miR-205/YAP1 axis.
背景
丙泊酚是一种常用的临床静脉麻醉剂。在过去几年中，研究表明丙泊酚不仅具有良好的麻醉效果，还具有一定的抗癌作用。然而，其在胃癌（SC）中的作用及相关机制仍在研究中。
目的
本研究旨在确定丙泊酚对SC的影响及其相关机制。
方法
购买的SC细胞分别用不同浓度（5、10和20μg/mL）的丙泊酚、miR-205过表达和YAP1抑制进行处理。然后，进行细胞计数试剂盒-8（CCK8）、Transwell和流式细胞术检测，以确定处理后细胞的生物学行为变化以及miR-205和YAP1的表达。
结果
丙泊酚（10μg/mL和20μg/mL）抑制SC细胞的生长并促进其凋亡，过表达miR-205或抑制YAP1也能产生相同的效果。此外，丙泊酚（10μg/mL和20μg/mL）上调SC细胞中miR-205的表达。双荧光素酶报告基因检测显示YAP1可被miR-205靶向调控，挽救实验显示抑制miR-205或过表达YAP1可减弱丙泊酚对SC细胞生物学行为的影响。
结论
丙泊酚可通过miR-205/YAP1轴强烈抑制SC细胞的增殖和侵袭并诱导其凋亡。
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