Brosi Richard, Bittl Robert, Engelhard Christopher
Fachbereich Physik, Freie Universität Berlin, Arnimallee 14, Berlin, 14195, Germany,
Methods Mol Biol. 2014;1146:341-60. doi: 10.1007/978-1-4939-0452-5_13.
Flavoproteins often employ radical mechanisms in their enzymatic reactions. This involves paramagnetic species, which can ideally be investigated with electron paramagnetic resonance (EPR) spectroscopy. In this chapter we focus on the example of flavin-based photoreceptors and discuss, how different EPR methods have been used to extract information about the flavin radical's electronic state, its binding pocket, electron-transfer pathways, and about the protein's tertiary and quaternary structure.
黄素蛋白在其酶促反应中常常采用自由基机制。这涉及顺磁物质，理论上可用电子顺磁共振（EPR）光谱对其进行研究。在本章中，我们将重点讨论基于黄素的光感受器这一实例，并探讨如何运用不同的EPR方法来获取有关黄素自由基的电子态、其结合口袋、电子转移途径以及蛋白质三级和四级结构的信息。
