Mizuta Masanobu, Hirano Shigeru, Hiwatashi Nao, Kobayashi Toshiki, Tateya Ichiro, Kanemaru Shin-Ichi, Nakamura Tatsuo, Ito Juichi
Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto.
Laryngoscope. 2014 Oct;124(10):E411-7. doi: 10.1002/lary.24733. Epub 2014 May 27.
OBJECTIVES/HYPOTHESIS: Reactive oxygen species (ROS) are associated with aging. Astaxanthin (AST) is a strong antioxidant and has been reported to prevent various ROS-induced diseases. In the current study, we investigated the effect of AST on age-associated histological and mRNA changes of vocal folds.
Prospective animal experiment with control.
Six-month-old Sprague-Dawley rats were fed on a normal powder diet with 0.01% (w/w) AST (aged AST-treated group) or without AST (aged sham-treated group). After 12 months of feeding, the larynges were harvested for histology, immunohistochemical detection of 4-hydroxy-2-nonenal (4-HNE), and quantitative real-time polymerase chain reaction for basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF). Thirteen-week-old rats were used as a young control group (young group).
The expression of 4-HNE, an oxidative stress marker, significantly increased in the two aged groups compared with the young group. Histological examination showed that the deposition of hyaluronic acid in the lamina propria (LP) was significantly reduced in the aged sham-treated group compared with the young group, but no significant difference was observed between the aged AST-treated group and the young group. There were no significant differences in the mRNA expression of bFGF and HGF between the aged AST-treated group and the young group, although the expression of these genes was significantly reduced in the aged sham-treated group as compared with the young group.
These results suggest that AST has the potential to attenuate age-associated changes of vocal folds.
目的/假设：活性氧（ROS）与衰老相关。虾青素（AST）是一种强大的抗氧化剂，据报道可预防各种由ROS引发的疾病。在本研究中，我们调查了AST对声带与年龄相关的组织学和mRNA变化的影响。
有对照的前瞻性动物实验。
给6个月大的斯普拉格-道利大鼠喂食含0.01%（w/w）AST的正常粉末饲料（老年AST处理组）或不含AST的饲料（老年假处理组）。喂养12个月后，取出喉部进行组织学检查、4-羟基-2-壬烯醛（4-HNE）的免疫组化检测，以及碱性成纤维细胞生长因子（bFGF）和肝细胞生长因子（HGF）的定量实时聚合酶链反应。13周大的大鼠作为年轻对照组（年轻组）。
与年轻组相比，两个老年组中氧化应激标志物4-HNE的表达显著增加。组织学检查显示，与年轻组相比，老年假处理组固有层（LP）中透明质酸的沉积显著减少，但老年AST处理组与年轻组之间未观察到显著差异。老年AST处理组与年轻组之间bFGF和HGF的mRNA表达无显著差异，尽管与年轻组相比，老年假处理组中这些基因的表达显著降低。
这些结果表明，AST有可能减轻声带与年龄相关的变化。
