Tateya Ichiro, Tateya Tomoko, Watanuki Makoto, Bless Diane M
Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Institute for Virus Research, Kyoto University, Kyoto, Japan; The Hakubi Center, Kyoto University, Kyoto, Japan.
J Voice. 2015 Mar;29(2):133-9. doi: 10.1016/j.jvoice.2014.07.021. Epub 2014 Dec 9.
OBJECTIVES/HYPOTHESIS: Vocal fold scarring is one of the most challenging laryngeal disorders to treat. Hyaluronic acid (HA) is the main component of lamina propria, and it plays an important role in proper vocal fold vibration and is also thought to be important in fetal wound healing without scarring. Although several animal models of vocal fold scarring have been reported, little is known about the way in which HA is maintained in vocal folds. The purpose of this study was to clarify the homeostasis of HA by examining the expression of hyaluronan synthase (Has) and hyaluronidase (Hyal), which produce and digest HA, respectively.
Experimental prospective animal study.
Vocal fold stripping was performed on 38 Sprague-Dawley rats. Vocal fold tissue was collected at five time points (3 days-2 months). Expression of HA was examined by immunohistochemistry, and messenger RNA (mRNA) expression of Has and Hyal was examined by real-time polymerase chain reaction and in-situ hybridization.
In scarred vocal folds, expression of Has1 and Has2 increased at day 3 together with expression of HA and returned to normal at 2 weeks. At 2 months, Has3 and Hyal3 mRNA showed higher expressions than normal.
Expression patterns of Has and Hyal genes differed between normal, acute-scarred, and chronic-scarred vocal folds, indicating the distinct roles of each enzyme in maintaining HA. Continuous upregulation of Has genes in the acute phase may be necessary to achieve scarless healing of vocal folds.
目的/假设：声带瘢痕形成是最难治疗的喉部疾病之一。透明质酸（HA）是固有层的主要成分，在声带正常振动中起重要作用，并且被认为在胎儿伤口愈合且无瘢痕形成过程中也很重要。尽管已经报道了几种声带瘢痕形成的动物模型，但对于HA在声带中的维持方式知之甚少。本研究的目的是通过检测分别产生和消化HA的透明质酸合酶（Has）和透明质酸酶（Hyal）的表达来阐明HA的稳态。
实验性前瞻性动物研究。
对38只Sprague-Dawley大鼠进行声带剥离。在五个时间点（3天至2个月）收集声带组织。通过免疫组织化学检测HA的表达，并通过实时聚合酶链反应和原位杂交检测Has和Hyal的信使核糖核酸（mRNA）表达。
在瘢痕化的声带中，Has1和Has2的表达在第3天与HA的表达一起增加，并在2周时恢复正常。在2个月时，Has3和Hyal3 mRNA的表达高于正常水平。
Has和Hyal基因的表达模式在正常、急性瘢痕化和慢性瘢痕化的声带中有所不同，表明每种酶在维持HA方面具有不同的作用。急性期Has基因的持续上调可能是实现声带无瘢痕愈合所必需的。
